FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_HEATR1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_HEATR1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC660462.61496160045511No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGC314329.799943111785343No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGATC990.9386555418602446No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAATC390.3697733952782782No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGGATC370.3508106570588793No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGAGC320.3034038115103821No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGAATC240.22755285863278657No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC210.19910875130368824No Hit
ATCTTCCTTTTTTATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC160.15170190575519105No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGCAAAGAGAGAGCAGGAGC150.1422205366454916No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAAGC140.13273916753579218No Hit
ATCTTCCTTTTTGATACCCATCATTTTATAAGTAAAGAGAGAGCAGGAGC120.11377642931639328No Hit
ATCTTCCTTTTTTATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC110.10429506020669384No Hit
ATCTTCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGCC110.10429506020669384No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAATC110.10429506020669384No Hit
ATTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGCC110.10429506020669384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTCC10300.0125.01
CTTTTTT152.1597274E-4125.07
TTCCTTT10350.0124.396144
TCTTCCT10350.0124.396142
CTTCCTT10350.0124.396143
TCCTTTT10450.0123.205755
CCTTTTT10400.0123.197116
TTTTTGA10250.0123.170738
CTTTTTG10250.0123.170737
TTTTGAT10300.0122.5728159
GAGCCTT5950.062.50000446-47
ACCCCGC201.35797E-462.50000416-17
TACCCCG201.35797E-462.50000414-15
ATACCCC201.35797E-462.50000414-15
GCATTTT10350.062.50000420-21
ATTTTAT10350.062.50000422-23
AGGAGCC5950.062.50000444-45
TGATGAT10300.062.552-53
TCCTCTG255.379132E-662.560-61
AATCCTT150.003439112662.546-47
Produced by FastQC (version 0.11.5)