FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_FNDC3A_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_FNDC3A_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16983
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG1628495.88411941353118No Hit
AATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT650.38273567685332394No Hit
GATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT530.312076782665018No Hit
GAATGGACAGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG250.14720602955897072No Hit
GAATGGACGGTTTATATCCCTATACAGAGGACCATGTCATACATACAAAG200.1177648236471766No Hit
GAATGGACGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT190.11187658246481776No Hit
GAATGGACGATTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG180.10598834128245893No Hit
GAATGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT170.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGA16750.0125.000011
AATGGAC16750.0125.000012
GACGGTT16800.0124.6279756
ATGGACG16750.0124.626883
ACGGTTT16750.0124.626887
GGTTTGT16700.0124.6257559
TGGACGG16700.0124.6257554
CGGTTTG16700.0124.6257558
GGACGGT16700.0124.6257555
ATCCTAT16600.062.50000472-73
ACAAAGT16650.062.50000444-45
GAGAAGC150.003457465462.50000422-23
TAAATTC16650.062.50000478-79
CAGCATC7900.062.50000468-69
TCAAGCT16600.062.50000490-91
CCTATAC16650.062.50000418-19
CCTATAA16600.062.50000474-75
AATTCTG16650.062.50000480-81
GAGGACC16650.062.50000426-27
AGAAGCT150.003457465462.50000424-25
Produced by FastQC (version 0.11.5)