FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_FNDC3A_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_FNDC3A_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10459
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG985694.23463046180323No Hit
AATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT310.2963954488956879No Hit
GATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT280.26771201835739555No Hit
GAATGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT160.152978296204226No Hit
GAATGGATGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG130.12429486566593365No Hit
GAATGGACGGTTTGTATGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGAA130.12429486566593365No Hit
GAATGGACGATTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG120.11473372215316952No Hit
GAATGGACGGTTTTATCCCTATACAGAGGACCATGTCATACATACAAAGT120.11473372215316952No Hit
GAATAGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG120.11473372215316952No Hit
GAATGGACGGTTTGTAGGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGAA110.1051725786404054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGG10150.0125.04
GGACGGT10150.0125.05
GAATGGA10200.0124.999991
ATGGACG10200.0124.999993
AATGGAC10200.0124.999992
GGTTTGT10300.0124.39329
CGGTTTG10300.0124.39328
ACGGTTT10300.0124.39327
GACGGTT10250.0124.390246
CAGCATC5200.062.50000468-69
GCATCCT5200.062.50000470-71
GTCAGCA5200.062.50000466-67
ACAAAGT10250.062.544-45
CCTATAA10150.062.574-75
GAGGACC10250.062.526-27
ACCATGT10250.062.530-31
GGACCAT10250.062.528-29
TATAAAT10150.062.576-77
TGTCATA10250.062.534-35
GAGTCAG5150.062.564-65
Produced by FastQC (version 0.11.5)