FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_FNDC3A_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_FNDC3A_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22401
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG2117194.50917369760279No Hit
AATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT730.32587830900406234No Hit
GATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT590.2633810990580777No Hit
GAATGGACGGTTTGTATGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGAA420.18749162983795367No Hit
GAATGGACGGTTTATATCCCTATACAGAGGACCATGTCATACATACAAAG360.16070711128967458No Hit
GAATGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT290.1294585063166823No Hit
GAATGGACAGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG290.1294585063166823No Hit
GAATGGACGGTTTGTATGGAATGAAGCTGGGGAAGGTCCAGATCGGAAGA270.12053033346725592No Hit
GAATGGACGATTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG250.11160216061782956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGA22050.0124.999991
AATGGAC22050.0124.4331052
ATGGACG22000.0124.147733
TGGACGG21900.0124.143844
GGACGGT21900.0124.143845
GGTTTGT22100.0123.8687749
ACGGTTT22100.0123.8687747
CGGTTTG22050.0123.866218
GACGGTT22000.0123.863646
TTGTATG201.3748872E-462.50000412-13
AGAAGCT201.3748872E-462.50000424-25
TGTATGG201.3748872E-462.50000412-13
ACAAAGT21900.062.544-45
GTATGGA150.003464751262.514-15
GAGAAGC150.003464751262.522-23
CCTATAC21900.062.518-19
TATACAG21900.062.520-21
TACAGAG21900.062.522-23
GAGGACC21850.062.526-27
TTGTAGG255.4800057E-662.512-13
Produced by FastQC (version 0.11.5)