FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_FNDC3A_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_FNDC3A_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43848
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG3502079.86681262543331No Hit
GAATGGACGGTTTGTATGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGAA7451.699051268016785No Hit
GAATGGACGGTTTGTAGGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGAA6551.493796752417442No Hit
GAATGGACGGTTTGTATGGAATGAAGCTGGGGAAGGTCCAGATCGGAAGA5471.2474913336982303No Hit
GAATGGACGGTTTGTAGGGAATGAAGCTGGGGAAGGTCCAGATCGGAAGA5141.1722313446451378No Hit
GAATGGACGGTTTGTATGGAAAGAGCTGGGGAAGGTCCAGATCGGAAGAG4040.9213647144681628No Hit
GAATGGACGGTTTGTAGGGAAAGAGCTGGGGAAGGTCCAGATCGGAAGAG2560.5838350665936872No Hit
GAATGGACGGTTTGTATGGAAAGACTGGGGAAGGTCCAGATCGGAAGAGC2460.5610290093048714No Hit
GAATGGACGGTTTGTAGGGAAAGACTGGGGAAGGTCCAGATCGGAAGAGC2440.5564677978471082No Hit
GAATGGACGGTTTGTAGGGAAAGATGAAGCTGGGGAAGGTCCAGATCGGA1890.43103448275862066No Hit
GAATGGACGGTTTGTATGGAAAGATGAAGCTGGGGAAGGTCCAGATCGGA1860.4241926655719759No Hit
GAATGGACGGTTTGTATGGAAAGAAGCTGGGGAAGGTCCAGATCGGAAGA1680.38314176245210724No Hit
GAATGGACGGTTTGTAGGGAAAGAAAGCTGGGGAAGGTCCAGATCGGAAG1600.36489691662105456No Hit
GAATGGACGGTTTGTATGGAAAGAAAGCTGGGGAAGGTCCAGATCGGAAG1520.3466520707900018No Hit
GAATGGACGGTTTGTAGGGAAAGAAGCTGGGGAAGGTCCAGATCGGAAGA1450.3306878306878307No Hit
GAATGGACGGTTTGTAGGGAAAGAATGAAGCTGGGGAAGGTCCAGATCGG1420.3238460135011859No Hit
AATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT1310.2987593504834884No Hit
GAATGGACGGTTTGTATGGAAAGAATGAAGCTGGGGAAGGTCCAGATCGG1270.28963692756796205No Hit
GAATGGACGGTTTGTATGGGAAAGAGAAGCTGGGGAAGGTCCAGATCGGA1130.2577084473636198No Hit
GAATGGACGGTTTGTATGGGAAAGAGCTGGGGAAGGTCCAGATCGGAAGA1060.24174420726144866No Hit
GATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT1030.23490239007480387No Hit
GAATGGACGGTTTGTATGGGAAAGACTGGGGAAGGTCCAGATCGGAAGAG1020.2326217843459223No Hit
GAATGGACGGTTTGTAGGGAAAGAAATGAAGCTGGGGAAGGTCCAGATCG1020.2326217843459223No Hit
GAATGGACGGTTTGTATGGAAAGAAATGAAGCTGGGGAAGGTCCAGATCG970.22121875570151434No Hit
GAATGGACGGTTTGTATGGGAATGAAGCTGGGGAAGGTCCAGATCGGAAG890.2029739098704616No Hit
GAATGGACGGTTTGTATGGGAAAGAAGCTGGGGAAGGTCCAGATCGGAAG510.11631089217296114No Hit
GAATGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAGT510.11631089217296114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGA43500.0125.000011
GGTTTGA152.2068758E-4125.09
AATGGAC43450.0124.8561552
ATGGACG43500.0124.568973
TGGACGG43400.0124.567974
GACGGTT43450.0124.424626
ACGGTTT43450.0124.424627
GGACGGT43450.0124.424625
CGGTTTG43400.0124.423968
GGTTTGT43350.0124.279119
GTATGGG451.4551915E-1162.50000414-15
ATGGGAA451.4551915E-1162.50000416-17
GAGGACC37150.062.50000426-27
ATGGAAA1800.062.50000416-17
TGTATCC37250.062.50000412-13
ATACATA37150.062.50000438-39
GAAAGAT451.4551915E-1162.50000418-19
TATGGGA451.4551915E-1162.50000414-15
GATGGGG150.003475838862.522-23
ATGGGGA150.003475838862.524-25
Produced by FastQC (version 0.11.5)