FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DISL3_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DISL3_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21566
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG1604574.39951775943615No Hit
TGGATGGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACA285313.229157006398962No Hit
TGGATGGAAACTTAAGCGTTGGTGACTGGAGTTGGTGTGGGCAATTGAGA3471.6090141890012055No Hit
TGGATGGAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG2881.335435407586015No Hit
TGGATGGAAACTTAAGCGTTGTGTGACTGGAGTTGGTGTGGGCAATTGAG2201.0201242696837616No Hit
TGGATGGAAACTTAAGCGTTGTGACTGGAGTTGGTGTGGGCAATTGAGAT1740.8068255587498842No Hit
TGGATGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG910.4219604933691922No Hit
TGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG830.3848650653806918No Hit
TGGATGGAAACTTAAGCGTGTGACTGGAGTTGGTGTGGGCAATTGAGATC530.24575721042381526No Hit
TGGATGGAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACAC450.20866178243531486No Hit
TGGATGGAAACTTAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG410.19011406844106463No Hit
TGGATGGAAACTTAAGCGGTGACTGGAGTTGGTGTGGGCAATTGAGATCG380.17620328294537696No Hit
TGGATGGAAATTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG350.16229249744968932No Hit
TGGTGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG340.15765556895112678No Hit
TGGATGGAAACTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG290.13447092645831402No Hit
GGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG290.13447092645831402No Hit
TGGATGGAAACTTAAACGTTGTTGATGATATTCCAGAATTCAAAGACTTG220.10201242696837615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAA204.359472E-6125.000012
GGATGGA20900.0125.02
GAACTTA301.7444108E-9125.07
TGGATGG20900.0125.01
GGAACTT301.7444108E-9125.06
ATGGAAC353.45608E-11124.999994
TGGAACT353.45608E-11124.999995
GATGGAA21050.0124.406173
ATGGAAA20750.0124.397594
TGGAAAC20650.0124.394685
GGAAACT20650.0124.394686
GAAACTT20900.0122.906697
AAACTTA21000.0122.321438
AACTTAA21300.0120.59869
ATGAAAC251.3216746E-5100.04
GATGAAA251.3216746E-5100.03
TGAAACT251.3216746E-5100.05
TGGCAAT201.374296E-462.50000490-91
AAGCGTG201.374296E-462.50000414-15
GTTGTGT201.374296E-462.50000418-19
Produced by FastQC (version 0.11.5)