FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DISL3_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DISL3_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG1606782.02052172137424No Hit
TGGATGGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACA17038.693654602072593No Hit
TGGATGGAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG2681.3681147582827098No Hit
TGGATGGAAACTTAAGCGTTGGTGACTGGAGTTGGTGTGGGCAATTGAGA1270.6483230384399408No Hit
TGGATGGAAACTTAAGCGTTGTGTGACTGGAGTTGGTGTGGGCAATTGAG1040.5309102047067231No Hit
TGGATGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG900.45944152330389504No Hit
TGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG840.4288120884169687No Hit
TGGATGGAAACTTAAGCGTTGTGACTGGAGTTGGTGTGGGCAATTGAGAT400.20419623257950892No Hit
TGGATGGAAACTTAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG390.1990913267650212No Hit
TGGATGGAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACAC370.18888151513604573No Hit
TGGATGGAAATTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG360.183776609321558No Hit
TGGATAGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG270.1378324569911685No Hit
TGGATGGAAACTTAAGCGTTTTTGATGATATTCCAGAATTCAAAGACTTG270.1378324569911685No Hit
GGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG270.1378324569911685No Hit
TGGTGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG260.13272755117668078No Hit
TGGATGGAAACTTAAGCATTGTTGATGATATTCCAGAATTCAAAGACTTG250.12762264536219306No Hit
TGGAGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG200.10209811628975446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGATGG19100.0125.01
TGATGGA152.1883627E-4124.999991
ATGGAAC353.45608E-11124.999994
GAACTTA353.45608E-11124.999997
TGGAACT353.45608E-11124.999995
GGAACTT353.45608E-11124.999996
GGATGGA19150.0124.673642
GATGGAA19300.0123.704663
ATGGAAA18950.0123.680734
TGGAAAC18850.0123.673745
GGAAACT18900.0123.3465656
GAAACTT19050.0122.7034157
AAACTTA19150.0122.062678
AACTTAA19450.0120.179959
GGTGAGA10050.062.50000450-51
CTTGGGT10050.062.50000446-47
TGGGTGA10050.062.50000448-49
GGAGAGC150.003461447862.499996104-105
AATCCAA201.3727033E-462.49999666-67
CGGAGAG150.003461447862.499996104-105
Produced by FastQC (version 0.11.5)