FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DISL3_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DISL3_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25400
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG1778070.0No Hit
TGGATGGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACA356214.023622047244094No Hit
TGGATGGAAACTTAAGCGTTGGTGACTGGAGTTGGTGTGGGCAATTGAGA5792.279527559055118No Hit
TGGATGGAAACTTAAGCGTTGTGACTGGAGTTGGTGTGGGCAATTGAGAT3981.5669291338582678No Hit
TGGATGGAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG3041.1968503937007875No Hit
TGGATGGAAACTTAAGCGTTGTGTGACTGGAGTTGGTGTGGGCAATTGAG2530.9960629921259843No Hit
TGGATGGAAACTTAAGCGTGTGACTGGAGTTGGTGTGGGCAATTGAGATC2330.9173228346456693No Hit
TGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG1110.437007874015748No Hit
TGGATGGAAACTTAAGCGGTGACTGGAGTTGGTGTGGGCAATTGAGATCG1010.3976377952755905No Hit
TGGATGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG970.38188976377952755No Hit
TGGATGGAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACAC600.23622047244094488No Hit
TGGATGGAAACTTAAGCGTTGACTGGAGTTGGTGTGGGCAATTGAGATCG500.19685039370078738No Hit
TGGATGGAAACTTAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG410.1614173228346457No Hit
TGGTGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG400.15748031496062992No Hit
TGGATGGAAACTTAAGCGTTTTTGATGATATTCCAGAATTCAAAGACTTG350.1377952755905512No Hit
TGGATGGAAATTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG320.12598425196850394No Hit
GGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG320.12598425196850394No Hit
TGGATGGAAACTTAAGCATTGTTGATGATATTCCAGAATTCAAAGACTTG260.10236220472440946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGAAC450.0125.000014
GAACTTA450.0125.000017
TGGAACT450.0125.000015
GGAACTT450.0125.000016
TGATGGA152.1960525E-4125.01
GGATGAA152.1960525E-4125.02
ATGAAAC152.1960525E-4125.04
GATGAAA152.1960525E-4125.03
TGAAACT152.1960525E-4125.05
GGATGGA24950.0124.74952
TGGATGG24950.0124.74951
GATGGAA25100.0124.0039753
ATGGAAA24650.0123.98584
TGGAAAC24550.0123.727095
GGAAACT24550.0123.727096
GAAACTT24650.0122.97167
AAACTTA24750.0122.474758
AACTTAA25150.0120.278339
CGTTGTG850.062.50000416-17
GGAGAGC255.4905995E-662.5104-105
Produced by FastQC (version 0.11.5)