FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DISL3_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DISL3_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31540
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG2205569.92707672796449No Hit
TGGATGGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACA436113.826886493341789No Hit
TGGATGGAAACTTAAGCGTTGGTGACTGGAGTTGGTGTGGGCAATTGAGA6412.0323398858592263No Hit
TGGATGGAAACTTAAGCGTTGTGACTGGAGTTGGTGTGGGCAATTGAGAT5551.7596702599873177No Hit
TGGATGGAAACTTAAGCGTTGTGTGACTGGAGTTGGTGTGGGCAATTGAG5491.7406467977171844No Hit
TGGATGGAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG3381.0716550412175017No Hit
TGGATGGAAACTTAAGCGTGTGACTGGAGTTGGTGTGGGCAATTGAGATC2480.7863031071655041No Hit
TGGATGGAAACTTAAGCGGTGACTGGAGTTGGTGTGGGCAATTGAGATCG1550.4914394419784401No Hit
TGGATGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG1300.4121750158528852No Hit
TGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG1170.3709575142675967No Hit
TGGATGGAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACAC650.2060875079264426No Hit
TGGATGGAAACTTAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG600.19023462270133165No Hit
TGGATGGAAATTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG530.16804058338617628No Hit
TGGATGGAAACTTAAGCGTGACTGGAGTTGGTGTGGGCAATTGAGATCGG520.1648700063411541No Hit
GGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG420.13316423589093215No Hit
TGGATGGAAACTTAAGCGTTGACTGGAGTTGGTGTGGGCAATTGAGATCG390.12365250475586556No Hit
TGGATGGAAACTTAAGCGTTTTTGATGATATTCCAGAATTCAAAGACTTG390.12365250475586556No Hit
TGGTGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTGG380.12048192771084339No Hit
TGGATGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACAC330.1046290424857324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAA204.3913024E-6125.000012
ATGAAAC152.2010376E-4125.04
GATGAAA152.2010376E-4125.03
ATGGAAC301.7698767E-9125.04
TGGAACT301.7698767E-9125.05
GGAACTT301.7698767E-9125.06
TGAAACT152.2010376E-4125.05
GGATGGA30700.0124.999992
TGGATGG30700.0124.999991
GATGGAA30950.0123.99033
ATGGAAA30600.0123.978764
TGGAAAC30600.0123.774515
GGAAACT30650.0123.57266
GAAACTT30800.0122.970787
AAACTTA30950.0122.3747948
AACTTAA31150.0121.3884359
GAACTTA355.173206E-9107.142857
TGATGGA251.3312925E-599.999991
CTCCATT201.3792221E-462.500004124-125
GCGTGTG201.3792221E-462.50000416-17
Produced by FastQC (version 0.11.5)