FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DENND5A_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DENND5A_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14966
Sequences flagged as poor quality0
Sequence length131
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT889459.42803688360283No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT387425.88534010423627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTTCT4112.7462247761592944No Hit
ATCTGCCTCATCACACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAA870.5813176533475879Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAATGCATGTCGGAGCGTGCT860.5746358412401443No Hit
ATCTGCCTCATCACACCCAGTCACTTCACCTCAGAGATCGGAAGAGCACA530.3541360416945076No Hit
ATCTGCCTCGTCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT420.2806361085126286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACTTCACCTCAGAGTCGGAAGAGCACACGTCTGAACTC390.26059067219029797Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATCTGCCTCATCACACCCTCTTTCCCTACACTTCACCTCAGAGATCGGAA290.1937725511158626No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAGAGCACACGTCTGAACTC270.18040892690097554Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTCCT250.16704530268608847No Hit
ATCTGCCTCATCACACTCTTTCCCTACACTTCACCTCAGAGATCGGAAGA250.16704530268608847No Hit
ATCTGCCTCATCACACCTCTTTCCCTACACTTCACCTCAGAGATCGGAAG240.16036349057864493No Hit
ATCTGCCTCATCACAGTCACTTCACCTCAGAGATCGGAAGAGCACACGTC190.12695443004142723No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGACACACGTCTGAACTC180.1202726179339837No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAAGCACACGTCTGAACTC180.1202726179339837No Hit
ATCTGCCTCATCACTCTTTCCCTACACTTCACCTCAGAGATCGGAAGAGC170.11359080582654016No Hit
ATCTGCCTCATCACACAGTCACTTCACCTCAGAGATCGGAAGAGCACACG150.10022718161165307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTCA14650.0125.000014
GCCTCAT14550.0125.05
CCTCATC14500.0125.06
CATCACT4350.0124.999999
TCTGCCT14800.0124.57772
ATCTGCC14800.0124.57771
CTGCCTC14750.0124.576283
CTCATCA14650.0124.146777
CATCACA10350.0123.792279
TCATCAC14750.0123.728828
ATCTGCT206.840622E-493.749991
GGCATGT9400.062.50000432-33
TGCTGGA2800.062.50000446-47
GCAGCAA6750.062.50000450-51
TGAAGGC9400.062.50000428-29
AAGGCAT9400.062.50000430-31
ATCACTT4300.062.50000410-11
CTGCAGC6750.062.50000448-49
TTATGCC150.003453463862.572-73
GAGCGTT255.4354805E-662.542-43
Produced by FastQC (version 0.11.5)