FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DENND5A_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DENND5A_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097
Sequences flagged as poor quality0
Sequence length131
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT713464.28764530954312No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT230820.79841398576192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTTCT3343.0098224745426694No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAATGCATGTCGGAGCGTGCT640.5767324502117689No Hit
ATCTGCCTCATCACACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAA540.48661800486618007Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGCCTCATCACACCCATCCTCTCCAGATGCCCAGCCTCATTGCTTCT350.31540055870956113No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAGAGCACACGTCTGAACTC280.25232044696764894Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTGCCTCATCACTTCACCTCAGAGTCGGAAGAGCACACGTCTGAACTC250.22528611336397225Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATCTGCCTCATCACACCCTCTTTCCCTACACGTCACTTCACCTCAGAGAT250.22528611336397225No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTCCT230.20726322429485444No Hit
ATCTGCCTCGTCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT220.1982517797602956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCTCTTTCCCTACACGTCACTTCACCTCAGAGATC200.18022889069117778No Hit
ATCTGCCTCATCACACCTCTTTCCCTACACTTCACCTCAGAGATCGGAAG180.16220600162206003No Hit
ATCTGCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG180.16220600162206003No Hit
ATCTGCCTCATCACACCCAAAGACCCCCAAATGTCTCATCCAATCACAGT150.13517166801838335No Hit
ATCTGCCTCATCACAGAATTTCAAGGCAGTCACTTCACCTCAGAGATCGG150.13517166801838335No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTACT130.11714877894926556No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAAGCACACGTCTGAACTC120.10813733441470669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTCA10800.0125.000014
CCTCATC10800.0125.000016
CTGCCTC10850.0124.999993
TCTGCCT10950.0124.4292142
ATCTGCC10950.0124.4292141
TCATCAC10850.0124.423968
CTCATCA10850.0124.423967
GCCTCAT10900.0123.853215
CATCACA8800.0123.5795449
CATCACT2100.0122.023819
GAGCGTT201.3595489E-462.50000442-43
TGGAGCA2700.062.50000450-51
GAGCAAC2700.062.50000452-53
CTGGAGC2700.062.50000448-49
GCGTTCT201.3595489E-462.50000444-45
AGCGTTC201.3595489E-462.50000442-43
GGAGCAA2700.062.50000450-51
CGTTCTG201.3595489E-462.50000444-45
CACACCT150.003441509862.512-13
TGCTGGA2600.062.546-47
Produced by FastQC (version 0.11.5)