FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DENND5A_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DENND5A_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18795
Sequences flagged as poor quality0
Sequence length131
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT1177162.628358606012235No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT410321.83027400904496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTTCT5663.011439212556531No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAATGCATGTCGGAGCGTGCT1150.6118648576749135No Hit
ATCTGCCTCATCACACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAA1110.590582601755786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGCCTCATCACACCCATCCTCTCCAGATGCCCAGCCTCATTGCTTCT890.4735301942005853No Hit
ATCTGCCTCGTCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT530.2819898909284384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTCCT370.1968608672519287No Hit
ATCTGCCTCATCACAGGCAGTCACTTCACCTCAGAGATCGGAAGAGCACA350.186219739292365No Hit
ATCTGCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG320.17025804735301941No Hit
ATCTGCCTCATCACTTCACCTCAGAGTCGGAAGAGCACACGTCTGAACTC310.16493748337323755Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATCTGCCTCATCACACCTCTTTCCCTACACTTCACCTCAGAGATCGGAAG300.1596169193934557No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAGAGCACACGTCTGAACTC280.14897579143389197Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTGCCTCATCACAGTCACTTCACCTCAGAGATCGGAAGAGCACACGTC270.14365522745411013No Hit
ATCTGCCTCATCACACCCAAAGACCCCCAAATGTCTCATCCAATCACAGT240.12769353551476456No Hit
ATCTGCCTATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG240.12769353551476456No Hit
ATCTGCCTCATCACAGAATTTCAAGGCAGTCACTTCACCTCAGAGATCGG220.11705240755520085No Hit
ATCTGCCTCATCACACCCTCTTTCCCTACACTTCACCTCAGAGATCGGAA200.10641127959563715No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTACT190.10109071561585528No Hit
ATCTGCCTCATCACACTCTTTCCCTACACTTCACCTCAGAGATCGGAAGA190.10109071561585528No Hit
ATCTGCCTCATCACACCCTCTTTCCCTACACGTCACTTCACCTCAGAGAT190.10109071561585528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGT152.1869395E-4125.05
TGCCTCG152.1869395E-4125.04
TGCCTCA18600.0125.04
TCATCAC18400.0125.08
TCTGCCT18750.0124.999992
CTGCCTC18750.0124.999993
CTCATCA18550.0124.999997
ATCTGCC18750.0124.999991
CCTCATC18650.0124.664876
GCCTCAT18700.0124.331565
CATCACA14300.0124.125879
CATCACT4200.0123.511919
CTTTCAT13350.062.50000422-23
TTCATGA13350.062.50000424-25
TCGGAGC13350.062.50000438-39
CGTGCTG12550.062.50000444-45
AGCGTGC12550.062.50000442-43
AGCAACT13350.062.50000452-53
GGAGCGT13350.062.50000440-41
GAGCGTT600.062.542-43
Produced by FastQC (version 0.11.5)