FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_DENND5A_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_DENND5A_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252
Sequences flagged as poor quality0
Sequence length131
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT892162.59472354757227No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT328623.056413134998596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTTCT4052.8417064271681167No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAATGCATGTCGGAGCGTGCT800.561324726354196No Hit
ATCTGCCTCATCACACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAA730.5122088127982037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGCCTCATCACTTCACCTCAGAGTCGGAAGAGCACACGTCTGAACTC370.2596126859388156Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATCTGCCTCATCACACCTCTTTCCCTACACTTCACCTCAGAGATCGGAAG350.2455795677799607No Hit
ATCTGCCTCATCACACCCATCCTCTCCAGATGCCCAGCCTCATTGCTTCT320.22452989054167838No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGTAAGAGCACACGTCTGAACT290.20348021330339602Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCTGCCTCGTCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT290.20348021330339602Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGCCTCATCACTTCACCTCAGAGATCGGAGAGCACACGTCTGAACTC240.16839741790625876Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTGCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG230.1613808588268313No Hit
ATCTGCCTCATCACACCCTCTTTCCCTACACTTCACCTCAGAGATCGGAA200.140331181588549No Hit
ATCTGCCTATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG170.11928150435026663No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTACT160.11226494527083919No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGACACACGTCTGAACTC150.10524838619141172No Hit
ATCTGCCTCATACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTG150.10524838619141172No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCAACGTCTGAACTC150.10524838619141172No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAAGCACACGTCTGAACTC150.10524838619141172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCACT4050.0125.09
CTGCCTA152.1759783E-4125.03
ATCTGCC14100.0125.01
TCTGCCT14150.0124.5583042
GCCTCAT14050.0124.110335
TGCCTCA14050.0124.110334
CTGCCTC14050.0124.110333
CCTCATC14000.0124.107146
CTCATCA14000.0123.660717
TCATCAC13950.0123.655918
CATCACA9900.0123.1060649
GAGCGTT358.682036E-962.50000442-43
TGCTGGA3550.062.50000446-47
GCGTTCT358.682036E-962.50000444-45
AGCGTTC358.682036E-962.50000442-43
CGTTCTG358.682036E-962.50000444-45
CCGCGAA150.003451799362.5116-117
CGCGAAA150.003451799362.5116-117
GTTCTGG150.003451799362.546-47
TCTGGAG150.003451799362.548-49
Produced by FastQC (version 0.11.5)