FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_CEP350_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_CEP350_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA727260.73665747932849No Hit
GAGATCCCCTGGTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA368330.7608786436148No Hit
GAGATCCCCTGGTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA900.7516913054372337No Hit
GAGATCCCCTGGTCCCTCTTTCCCTACACGACGCTCTGATATGCAAGCCT790.6598179236615719Illumina Single End PCR Primer 1 (95% over 24bp)
GAGATCCCCTGGTCCCAAATCTTTCCCTACACGACGCTCTGATATGCAAG750.6264094211976947Illumina Single End PCR Primer 1 (95% over 23bp)
GAGATCCCCTGTTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA670.5595924162699407No Hit
GAGATCCCCTGTTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA650.5428881650380022No Hit
GAGATCCCCTGGTCCCAAACTCTTTCCCTACACGACGCTCTGATATGCAA380.317380773406832Illumina Single End PCR Primer 1 (96% over 25bp)
GAGATCCCCTGGTCCCATCTTTCCCTACACGACGCTCTGATATGCAAGCC250.2088031403992316Illumina Single End PCR Primer 1 (95% over 22bp)
GAGATCCCCTGGTCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAAG210.17539463793535454No Hit
GATATGCAAGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC190.158690386703416Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGATCCCCTGTTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA140.1169297586235697No Hit
GAGATCCCCTGGTCCCAATCTTTCCCTACACGACGCTCTGATATGCAAGC120.10022550739163118Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT11850.0125.000014
GATCCCC11850.0125.000013
AGATCCC11900.0125.000012
TCCCCTG11850.0125.000015
GAGATCC11900.0125.000011
CCCCTGG11750.0125.06
CTGGTCC11800.0124.470349
CCCTGGT11800.0124.470347
CCTGGTC11800.0124.470348
AAACCAT201.3618215E-462.50000416-17
AAACCAC3550.062.50000416-17
ATAAGGG201.3618215E-462.50000422-23
ACCACAA3550.062.50000418-19
ACCATAA201.3618215E-462.50000418-19
CACAAGG3550.062.50000420-21
AACCATA201.3618215E-462.50000418-19
GAGCAAC201.3618215E-462.50000498-99
AACCACA3550.062.50000418-19
AGAGCAA201.3618215E-462.50000496-97
CCACAAG3550.062.50000420-21
Produced by FastQC (version 0.11.5)