FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_CEP350_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_CEP350_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10251
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA604058.921080870159No Hit
GAGATCCCCTGGTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA329432.13345039508341No Hit
GAGATCCCCTGGTCCCTCTTTCCCTACACGACGCTCTGATATGCAAGCCT1111.082821188176763Illumina Single End PCR Primer 1 (95% over 24bp)
GAGATCCCCTGGTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA630.6145741878841089No Hit
GAGATCCCCTGTTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA580.5657984586869573No Hit
GAGATCCCCTGTTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA580.5657984586869573No Hit
GATATGCAAGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC510.4975124378109453Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGATCCCCTGGTCCTCTTTCCCTACACGACGCTCTGATATGCAAGCCTG250.24387864598575748Illumina Single End PCR Primer 1 (95% over 23bp)
GAGATCCCCTGGTCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAAG200.19510291678860597No Hit
GAGATCCCCTGGTCCCATCTTTCCCTACACGACGCTCTGATATGCAAGCC170.16583747927031509Illumina Single End PCR Primer 1 (95% over 22bp)
GAGATCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAAG170.16583747927031509No Hit
GAGATCCCCTGGTCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAAG150.1463271875914545No Hit
GAGATCCCCTGGTCCCAAACTCTTTCCCTACACGACGCTCTGATATGCAA130.1268168959125939Illumina Single End PCR Primer 1 (96% over 25bp)
GAGATCCCCTGGTCCCAATCTTTCCCTACACGACGCTCTGATATGCAAGC120.11706175007316359Illumina Single End PCR Primer 1 (95% over 21bp)
GAGATCCCCTGGTCTTTCCCTACACGACGCTCTGATATGCAAGCCTGTAG110.1073066042337333Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCC10050.0125.000011
AGATCCC10150.0124.384242
ATCCCCT10100.0124.3811954
GATCCCC10100.0124.3811953
TCCCCTG10100.0124.3811955
CCCTGGT10000.0124.3757
CCCCTGG10000.0124.3756
CTGGTCC9950.0124.371869
CCTGGTC9950.0124.371868
CGCTGAA150.003437938262.5104-105
ACACGCT150.003437938262.5102-103
AAACCAT150.003437938262.516-17
AAACCAG5950.062.516-17
TGGCACA9900.062.532-33
ACGCTGA150.003437938262.5104-105
TCCCAAA9800.062.512-13
CACACGC150.003437938262.5100-101
TCTAGCT9900.062.564-65
ATAAGGG150.003437938262.522-23
CACGCTG150.003437938262.5102-103
Produced by FastQC (version 0.11.5)