FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_CEP350_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_CEP350_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17469
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA1039559.50540958268934No Hit
GAGATCCCCTGGTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA551531.570210086438834No Hit
GAGATCCCCTGGTCCCTCTTTCCCTACACGACGCTCTGATATGCAAGCCT1580.9044593279523727Illumina Single End PCR Primer 1 (95% over 24bp)
GAGATCCCCTGGTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA1380.7899708054267559No Hit
GAGATCCCCTGGTCCCAAATCTTTCCCTACACGACGCTCTGATATGCAAG970.5552693342492415Illumina Single End PCR Primer 1 (95% over 23bp)
GAGATCCCCTGTTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA930.5323716297441181No Hit
GAGATCCCCTGTTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA870.49802507298643306No Hit
GATATGCAAGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC460.2633236018089187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGATCCCCTGGTCCCAAACTCTTTCCCTACACGACGCTCTGATATGCAA450.2575991756826378Illumina Single End PCR Primer 1 (96% over 25bp)
GAGATCCCCTGGTCCCATCTTTCCCTACACGACGCTCTGATATGCAAGCC370.2118037666723911Illumina Single End PCR Primer 1 (95% over 22bp)
GAGATCCCCTGGTCCCAATCTTTCCCTACACGACGCTCTGATATGCAAGC360.20607934054611027Illumina Single End PCR Primer 1 (95% over 21bp)
GAGATCCCCTGGTCCTCTTTCCCTACACGACGCTCTGATATGCAAGCCTG290.16600835766214436Illumina Single End PCR Primer 1 (95% over 23bp)
GAGATCCCCTGTTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA220.12593737477817848No Hit
GAGATCCCCTGGTCCTAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA180.10303967027305513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTC204.3358996E-6125.000018
CCCTGTT204.3358996E-6125.000017
CCCCTGT204.3358996E-6125.000016
CCCCTGG16850.0125.000016
CTGTTCC204.3358996E-6125.000019
ATCCCCT17150.0124.999994
GATCCCC17150.0124.999993
AGATCCC17200.0124.999992
TCCCCTG17150.0124.999995
GAGATCC17200.0124.999991
CCTGGTC16950.0124.631278
CTGGTCC16900.0124.630189
CCCTGGT16850.0124.629097
CGCTGAA150.00345832362.500004104-105
GGAGAGC201.3706394E-462.50000494-95
TGGCACA16600.062.50000432-33
TCCCAAA16600.062.50000412-13
CGGAGAG201.3706394E-462.50000492-93
CACACGC150.00345832362.500004100-101
CACGCTG150.00345832362.500004102-103
Produced by FastQC (version 0.11.5)