FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_CEP350_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_CEP350_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA1084361.85396463205932No Hit
GAGATCCCCTGGTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA577632.94922989161437No Hit
GAGATCCCCTGGTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA1250.713063320022818No Hit
GAGATCCCCTGTTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA950.5419281232173417No Hit
GAGATCCCCTGTTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA910.5191100969766115No Hit
GAGATCCCCTGGTCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAAG250.1426126640045636No Hit
GAGATCCCCTGTTCCCAAACCATAAGGGCTACTGGCACAGTTATGTAAAA220.12549914432401596No Hit
GAGATCCCCTGGTCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAAG200.11409013120365087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTC353.45608E-11125.000018
CCCTGTT353.45608E-11125.000017
CCCCTGT353.45608E-11125.000016
CTGTTCC353.45608E-11125.000019
ATCCCCT17150.0125.04
GATCCCC17250.0125.03
AGATCCC17350.0125.02
TCCCCTG17150.0125.05
GAGATCC17350.0125.01
CCCTGGT16800.0124.255957
CCCCTGG16800.0124.255956
CCTGGTC16800.0124.255958
CTGGTCC16750.0124.253739
CTATATC150.00345840662.500004124-125
GTTCCCA403.5288394E-1062.50000410-11
TGTTCCC403.5288394E-1062.50000410-11
TATGATC302.1813503E-762.500004124-125
TCGGAAA302.1813503E-762.50000492-93
GAAGACA201.3706944E-462.50000494-95
GCTATCT150.00345840662.500004124-125
Produced by FastQC (version 0.11.5)