FastQCFastQC Report
Fri 31 Jan 2020
000000000-D6VGL_l01_n01_HiDEF-seq-pilot1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D6VGL_l01_n01_HiDEF-seq-pilot1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406203
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACA1358.4074505E-426.6666662
TAGATCG1708.662218E-48.470589140-144
AGATCGG4651.7710112E-45.2645164140-144
Produced by FastQC (version 0.11.5)