FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492070
Sequences flagged as poor quality0
Sequence length287
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT251691.6868511530960344No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT218281.4629340446493797No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT206781.3858599127386784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACA48450.0275.426036
TGATGAC48350.0274.843325
GCTATCG38600.0273.897283
TGACAGC48750.0273.73118
GGCGATA33050.0273.530981
CTATCGA39700.0272.974674
CCGCTAT39300.0272.56311
GCGATAC33850.0272.4162
CGCTATC39600.0268.739472
TATCGAC40550.0266.56565
GATACGA35150.0265.90744
ATGACAG51350.0261.227637
TACGACA36700.0258.852026
TCGACAG48600.0221.839047
GTACTGA76950.0175.950671
CGCGATA950.0175.950651
CGACAGC124750.0174.857598
TACTGAT79600.0169.743032
CGATACG55050.0169.025563
GCTAACG1650.0160.399463
Produced by FastQC (version 0.11.5)