FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446307
Sequences flagged as poor quality0
Sequence length287
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9746921.839003197350703No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9451721.177575077245038No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT7500016.80457622219683No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG35760.8012421942743448No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG21790.4882289545088919No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC20430.4577566562926416No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG20310.4550679240970901No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG20070.44969045970598714No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC17970.402637646283836No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT17660.3956917547786613No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC17340.3885218022571907No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16280.36477133452981914No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG14420.323095985498771No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC13260.2971049076084399No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACATCCTATG13250.296880846592144No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT13140.2944161754128884No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAATTCCACCA12430.2785078432558754No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGC11200.2509483382514726No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG10370.23235127389890814No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGTCATGGGCGAGCGC10280.2303347247522445No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG9960.2231647722307739No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG9750.21845949088855876No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG9310.20860080617153662No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT9240.20703237905746494No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG8370.18753907063971662No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGATGGGCGAGC8150.18260972828120553No Hit
GTACTGATGACAGCAGTACTACTGACGATGGGACAGCAGTGTGTGAGGCT8070.18081724015083786No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC7770.17409540966195916No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG7590.17006231136863192No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACATATGGGA7300.16356454189604913No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGGCGAGCGCTATGAGCGAC7160.16042768766790572No Hit
GTACTGATGACAGCAGTATCTGCTGTCGATATCTGCTCGCCGATATATGG6960.15594646734198656No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG6820.15280961311384317No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACGATACTGC6610.14810433177162804No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCAGTGCGA6510.14586372160866848No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAACTATGGGC6450.14451935551089273No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG6340.1420546843316372No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG6340.1420546843316372No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGATCATCAGCATGGATCGA6330.14183062331534124No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCAGTATCATCGAGC6280.14071031823386146No Hit
CCGCTATCGACAGCAGTATCTGCTGTCAGTATTATCCGTCTATACGGATA6280.14071031823386146No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT6160.13802158603830997No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC6160.13802158603830997No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG5860.1312997555494312No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA5860.1312997555494312No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC5830.13062757250054335No Hit
GTACTGATGACAGGACAGCAGTGTGTGAGGCTATGGGAGAGCATAGACAT5730.12838696233758376No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT5730.12838696233758376No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC5700.1277147792886959No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG5610.12569823014203227No Hit
CTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGCTATGGGAGAGCG5530.12390574201166461No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA5520.12368168099536865No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG5520.12368168099536865No Hit
GTACTGATGACAGTACTATGGGAGAGCGCGCTCGTCTATGGGTCAGCAGT5350.11987264371833738No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGGAC5240.11740797253908183No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCACTA5210.11673578949019396No Hit
GTACTGATGACAGCAGTATCTGCTGTGGGCAGTGGCGAGCGCTATGAGCG5200.116511728473898No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT5130.11494330135982629No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT5000.11203050814797887No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGGGA4980.11158238611538696No Hit
GGCGATACGACCGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT4950.11091020306649908No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGATC4900.10978989798501929No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGATCGACAAATGGCAGTATT4840.10844553188724354No Hit
CCGCTATCGACAGCAGTATCTGCTGTCTATGGGAGAGCGCGCTCGTCGAC4780.1071011657894678No Hit
GTACTGATGACAACAGCTCGTCGACTATGGGTCAGCAGTACTACTGACGA4760.10665304375687588No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG4740.10620492172428396No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC4740.10620492172428396No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC4730.10598086070798801No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACTCGCCTAT4700.10530867765910013No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG4690.10508461664280418No Hit
GTACTGATGACAGCAGTATCTGCCGAGCCCATCTGGGCGAGCGCTATGAG4580.10261994546354863No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC4550.10194776241466076No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA4510.10105151834947693No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC4490.10060339631688502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA129250.0277.564731
CGATACG130150.0277.358063
GTACTGA167450.0277.1451
GCGATAC129850.0277.140472
GATACGA130200.0276.93054
GATGACA164800.0276.867286
CCGCTAT121500.0276.349271
TGATGAC166350.0276.21385
ATGACAG164900.0276.023477
TGACAGC163150.0275.99548
CTATCGA121150.0275.652654
GCTATCG121300.0275.311773
ATACGAC131150.0275.03085
CTGATGA167250.0274.810764
ACTGATG167450.0274.565733
CGCTATC122850.0272.972232
TATCGAC122300.0272.946725
TACTGAT168450.0272.439542
TACGACA131800.0271.348886
TCGACAG121800.0270.978827
Produced by FastQC (version 0.11.5)