FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220758
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6323328.64358256552424No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT4830221.88006776651356No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT4187218.967376040732386No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG8540.386848947716504No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC7580.3433624149521195No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC7060.3198072097047446No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC6890.31210646952771814No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG6450.29217514201070854No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG6100.27632067694036No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC6090.2758676922240644No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG5460.247329655097437No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT5440.24642368566484568No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA5330.24144085378559327No Hit
GTACTGATGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT5150.23328712889227118No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA4890.2215095262685837No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA4740.21471475552414862No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG4390.19886029045380008No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT4200.1902535808441823No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG3930.17802299350419917No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG3850.1743991157738338No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC3850.1743991157738338No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA3820.1730401616249468No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG3820.1730401616249468No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG3770.17077523804346842No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTACTAT3760.17032225332717274No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTATGCTATGGGAGAGC3740.1694162838945814No Hit
GTACTACTATGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTACTACT3700.16760434502939872No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT3690.16715136031310301No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT3660.16579240616421603No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG3610.16352748258273767No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC3530.1599036048523723No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG3490.1580916659871896No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGAGAGCGCGCTCGTC3440.15582674240571123No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG3430.15537375768941555No Hit
CTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTATCATCGACTAT3370.15265584939164153No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA3320.15039092581016317No Hit
GTACTGATGACAGCAGTATCCTATGGGAGAGCGCGCTCGTCGACTATGGG3300.14948495637757184No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGACTATGGGCGACTATGGGAC3290.14903197166127616No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG3280.14857898694498048No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA3210.14540809393091075No Hit
GTACTGATGCTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTATC3190.14450212449831942No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG3150.14269018563313673No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG2990.13544243017240598No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG2970.13453646073981465No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG2950.1336304913072233No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG2910.1318185524420406No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCACG2820.12774168999537955No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA2820.12774168999537955No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG2790.12638273584649254No Hit
GGCGATACGACAGCAGTATCTACTATGGCTATGGGAGAGCGCGCTCGTCG2730.1236648275487185No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT2720.12321184283242284No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG2700.1223058733998315No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACATGGGACTATG2620.11868199566946612No Hit
GTACTGCTATGGGCGAGCGCTATGAGCGACTATGGGACCTATGGGAGAGC2600.11777602623687478No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATTCTATGGGAGAGCATAGACAT2560.11596408737169209No Hit
GTACTGATGACAGCAGTATCTGCTGTCTCCCATATGGGAGAGCTATGGGT2560.11596408737169209No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA2550.1155111026553964No Hit
GGCTATGGCTATGGCGACTATGACCTATGGGTCAGCAGTACTACTGACGA2530.11460513322280505No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT2500.11324617907391805No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACGATGGGA2470.11188722492503102No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC2430.11007528605984833No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCGCTATGA2430.11007528605984833No Hit
GGCTATGGGAGAGCATAGACATCGAAGTATGGGACTACAGTCGCTACGAC2370.10735737776207431No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACACTATGGGCGAGCGCC2280.10328051531541325No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG2220.10056260701763922No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG2220.10056260701763922No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT2210.10010962230134356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT63150.0277.988431
CGATACG56400.0277.90923
GGCGATA56200.0277.906561
GATGACA85750.0277.512946
TACTGAT86700.0277.2042
TGACAGC86150.0277.194768
ATGACAG85950.0277.191387
GCTATCG63000.0277.102233
GTACTGA87250.0276.734071
ACTGATG86800.0276.724123
GATACGA56750.0276.195224
CGCTATC63750.0275.809142
CTGATGA86800.0275.600524
TCGACAG63250.0275.56647
TGATGAC86900.0275.443735
CGACAGC120300.0275.291668
GCGATAC56750.0275.21322
CTATCGA63600.0274.926184
ATACGAC57100.0274.502265
TACGACA57400.0272.339426
Produced by FastQC (version 0.11.5)