FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948203
Sequences flagged as poor quality0
Sequence length287
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT64678621.938312931640052No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT55324818.76560060484302No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT48227516.358269766362767No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAATTTTCTCTTAA135170.45848267571805607No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT124810.42334262599963435No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG103830.35218063342313943No Hit
CCGCATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATTAAA101620.3446845417361016No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT98450.3339322292257351No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG97790.3316935774096967No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT96870.3285730324540067No Hit
CTGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT73430.24906697401773215No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC70090.23773803906990124No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTACTAT69970.23733101146698513No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG66030.2239669385045738No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC62960.21355381566330406No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG62400.21165435351636233No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG60160.2040565049285955No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT57470.19493230282989332No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTATGCTATGGGAGAGC57280.19428784245860953No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA53100.180109714290366No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA53010.17980444358817896No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA52750.17892255044852745No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG52050.17654822276485033No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC51940.1761751141288439No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG51670.17525930202228274No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG51330.17410605714735383No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT46240.15684130299033003No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC43050.1460211525461442No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG42670.1447322318035766No Hit
CCGCTATCGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG42550.14432520420066053No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT41800.141781281682435No Hit
GTACTGATGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT40890.13869465569365474No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGAGTATG39520.13404775722702947No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAA39300.13330153995501667No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACGATGGGA38680.13119856400661692No Hit
GGCGATACGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACAGTA38630.13102896917206855No Hit
TTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT38560.1307915364037008No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG38030.12899383115748814No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCATGG37640.1276709914480109No Hit
GTACTGAATGTGATCATTAGGAAGATTACGAAAAATATAAAAAATATGAG37210.1262124758708949No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG36150.12261706537846954No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA35810.12146382050354063No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGGGAAAGCATAGACA35730.12119246876826324No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG35360.11993746699260532No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG35250.11956435835659891No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG34360.11654557030163798No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC34180.11593502889726386No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGACAGAGATATCATGCAAG33710.1143408374525092No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC33350.11311975464376096No Hit
GGCGATACGACAGCAGTATCTATGGGACAGCAGTATCATGGCAGCAGTAT32540.11037231832407741No Hit
GTACTGCTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT32410.10993137175425165No Hit
GTACTGATGCTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTATC32070.10877812687932276No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGAGTATGGGACTACAGTCG31360.10636988022873595No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG31220.10589501469200052No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC31120.10555582502290378No Hit
GGCGATACGACAGCAGTATCTGGATGGATCAGTATGGATCGACAAATGGC30980.10508095948616836No Hit
CCGCTATCGTATGGGACTACAGTCGCTACGACTATGGGACAGAGATATCA30930.10491136465161999No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG30520.10352068700832337No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGTATGGATGTA29870.1013159541591946No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT29820.10114635932464623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA781850.0278.173551
TGATGAC975300.0277.715365
GATGACA972100.0277.511546
CCGCTAT864750.0277.431851
GCGATAC793700.0277.268132
GTACTGA1001750.0277.059081
ACTGATG986750.0276.24383
TGACAGC985100.0276.211468
CGCTATC870700.0275.840062
TACTGAT992500.0275.822662
CTGATGA985700.0275.20934
GCTATCG878350.0274.912963
CTATCGA888550.0274.030884
CGACAGC1738350.0272.78928
CGATACG829500.0271.970343
TCGACAG886000.0271.92597
GATACGA838950.0270.733764
TATCGAC896250.0270.479525
ATACGAC841150.0270.307255
TACGACA845400.0268.99786
Produced by FastQC (version 0.11.5)