FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489588
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT13299027.1636559719601No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9921120.264181311633457No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9285618.966151131155176No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC33930.6930316919532341No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC20480.4183109063130633No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG18370.3752134447739732No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG16620.33946910463491753No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT15960.325988382068188No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG15680.32026928764593904No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG15600.3186352606681536No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC12920.2638953569123426No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG12540.25613372876786195No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC12000.2451040466678105No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG11920.2434700196900251No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC11580.23652540503443711No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA11270.23019355049551868No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT10750.21957237513991357No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA10280.20997246664542432No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT10210.20854269303986211No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG9740.19894278454537284No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA9640.1969002508231411No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG9480.19363219686757027No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG8790.1795387141841712No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC8780.179334460811948No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG8660.1768834203452699No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG8600.17565790011193086No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC8380.171164325923021No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG8260.16871328545634287No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG8250.16850903208411971No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG8130.16605799161744159No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTACAGTCGCTACGACT7930.1619729241729781No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT7580.15482405614516698No Hit
GTACTGATGACAGCAGTATCTCTATGGGAGAGCGCGCTCGTCGACTATGG6920.14134333357843737No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG6720.13725826613397385No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCGCTAT6640.13562423915618846No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC6630.1354199857839653No Hit
CCGCTATCGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA6590.1346029722950726No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG6560.13399021217840307No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT6550.13378595880617988No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA6490.13256043857284083No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG6350.1297008913617164No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT6340.1294966379894932No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG6270.12806686438393097No Hit
GTACTGCTATGGGCGAGCGCTATGAGCGACTATGGGACCTATGGGAGAGC6170.12602433066169924No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT6160.12582007728947606No Hit
TGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTACTACTGACGATGGG6060.1237775435672443No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG5850.11948822275055762No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA5850.11948822275055762No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG5790.11826270251721856No Hit
CCGCTATCGACAGCAGTATCTATGGGAGAGCATAGACATCGAGTATGGGA5770.11785419577277222No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA5650.1154031553060941No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG5640.11519890193387092No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG5560.11356487495608551No Hit
TTCGCCTATGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTACTACTG5030.10273944622825723No Hit
GTACTGATGACAGCAGTATCTGCTGGAGCAGTATCTGCTCGCACTATGGG4990.10192243273936452No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACATGGGACTATG4980.10171817936714135No Hit
GTACTGATGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG4960.10130967262269501No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG4960.10130967262269501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACA194800.0277.62096
CCGCTAT133150.0277.47121
GGCGATA126750.0277.30321
GATACGA126650.0276.972174
CGATACG126900.0276.755863
TGACAGC196600.0276.70898
TGATGAC195950.0276.560335
GCTATCG132500.0276.51933
GTACTGA197350.0276.433781
GCGATAC127000.0276.318542
ATGACAG196200.0275.85297
CTGATGA197000.0275.6524
ACTGATG197350.0275.37493
CTATCGA133150.0275.16944
TACTGAT197700.0274.605562
ATACGAC127900.0274.5925
TCGACAG134750.0272.00557
CGACAGC264500.0271.564368
TACGACA129400.0270.655336
CGCTATC136200.0270.13252
Produced by FastQC (version 0.11.5)