FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6853314
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT101716014.841870662864713No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT97151914.17590088532351No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT90804013.249648272354076No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG419090.6115143710035758No Hit
GACAGCAGTATCATCGACAGCAGTGTGTGTTGTTGTTTTGTTGTTAACTT380650.5554247186105875No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG333450.48655292899172575No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC307280.4483670236034713No Hit
GACAGCAGTATCATCGAATAATAAAACTTAATATCTATTCAAATTAATAA305690.4460469781480901No Hit
GACAGCAGTATCATCGAGAGACAGTATGGAGTCGAGAGCGCGCTCGTGGA291460.4252833008964714No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG212730.3104045721529759No Hit
GACAGCAGTATCATCTATGGAGTCGTCAGCAGTACTACTGAGTACTACTA203710.2972430564249646No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACGTGGTG203150.2964259334972832No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG192630.2810756956415539No Hit
GACAGCAGTATCATTGACTCTATTCAAATTAATAAATAAACCTCGATATA192130.28034612159898115No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC185100.27008831056040916No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT182420.26617779369221956No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC179430.26181494091763485No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG173550.25323515017698006No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGATCGACAAATGGCAGTATT159170.23225260071258955No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG156100.22777301609119327No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGA152780.2229286444485106No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGATC147350.21500547034617123No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG146200.213327450048254No Hit
GACAGCAGTATCATCGACAATGGATCGACAAATGGCAGTATTCATCCACA145330.21205799121417754No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT130370.19022913586040274No Hit
GTACTGATGACAGCAGTATCATCATATAAATAAATAAACATAAATAAATT129470.1889159025837719No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG126680.18484487942621627No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA124100.18108027736654123No Hit
GACAGCAGTACTCCACATACATTATACGAAGTTATGCTAGCCTAGTTCCA123770.18059875849844323No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGTCATGGGCGAGCGC121670.17753454751963793No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACATCCTATG119510.17438278765572393No Hit
GACAGCAGTATCATCGATGGATACGATCGACAAATGGCAGTATTCATCCA115250.16816681681300463No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC114610.1672329620385116No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG111990.1634099940554307No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGATCATCAGCATGGATCGA108420.1582008353914617No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG106540.1554576369913884No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG105410.15380879965517413No Hit
GACAGCAGTATCATCGAACCTCCCACACCTCCCCCTGAACCTGAAACATA104440.1523934260125831No Hit
GACAGCAGTATCATCGAGCGATGATCGTGGATCATGGATCGACAAATGGC104130.15194109010618803No Hit
GTACTGATGACAGCGCGCTGGGACAGAGATATCATGCGGTATGGATCGAC101040.14743232252308883No Hit
GACAGCAGTATCATCGAGTCGATCATGTATCATCGATGCTAGCCTAGTTC100390.14648387626774434No Hit
GTACTGATGACAGCAGTATCTGCTGTATCCATGTGGCCATACAGTTGTAA99610.14534574076133094No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCAGTGCGA99220.14477667300812425No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGAGAGCGCGCT98730.14406169044640302No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG94360.1376852133143177No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG93980.13713073704196246No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAATTCCACCA89040.12992254550134433No Hit
GACAGCAGTATCATCGCGAGCGCTATGGATCGACAAATGGCAGTATTCAT88980.1298349966162356No Hit
GACAGCAGTATCATCGAAATAAAGCATTTTTTTCACTGCATTCTAGTTGT85930.1253845949565422No Hit
GTACTGATGACAGCAGTACTACTGACGATGGGACAGCAGTGTGTGAGGCT85800.12519490570547329No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACATATGGGA84790.12372116613947647No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACACTATGGGCGA83890.12240793286284561No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGC83660.12207232880326217No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG81730.11925617299893161No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA81230.11852659895635893No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCAGTATCATCGAGC80700.11775325047123188No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC79620.11617737053927486No Hit
CGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT79560.11608982165416613No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAACTATGGGC79200.1155645283435138No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGGAC79150.11549157093925654No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGGCGAGCGCTATGAGCGAC76430.11152268814766111No Hit
CCGCTATCGACAGCAGTATCTGCTGAAACTCCGCTTGAGTTCCGCATCCG76420.11150809666680965No Hit
GGCGAAGTATGGATCGACAAATGGCAGTATTCATCCACAGCTTTAAGGCC76090.11102657779871168No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC75900.11074933966253406No Hit
CCGCTATCGACAGCAGTATCTGCTGTCAGTATTATCCGTCTATACGGATA75580.11028241227528754No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC74840.10920264269227997No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA74170.10822501347523257No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT73220.10683882279434447No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA69550.10148374932186091No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCATGGGCGAGCGCT69400.10126487710908912No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT69180.10094386453035713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC1423300.0277.599182
GGCGATA1414750.0277.55331
GCTATCG1682900.0276.97053
TGATGAC1979200.0276.824525
GATGACA1981150.0276.573156
CCGCTAT1709700.0276.141481
GTACTGA1999350.0275.296051
TGACAGC1965800.0275.238348
CTATCGA1697050.0275.104464
CGATACG1455800.0272.272923
CGCTATC1726650.0271.566562
TATCGAC1711250.0271.559575
TACTGAT2024500.0271.304842
ATACGAC1461400.0270.51455
ATGACAG2027800.0269.887577
GATACGA1476750.0269.184024
TACGACA1467900.0268.016176
GCTAACG14600.0236.683823
ACTGATG2363600.0232.953223
CTGATGA2374700.0231.658954
Produced by FastQC (version 0.11.5)