FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n02_JRS_019_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n02_JRS_019_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043165
Sequences flagged as poor quality0
Sequence length287
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT140898323.315315732732763No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT116801919.327934947994965No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT105210217.409784442423796No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC247660.40981836504546876No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG213550.3533744321063549No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG199610.33030704936899785No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG183440.30354954729847683No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC180510.2987010945423466No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT179490.29701323726888146No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC169960.281243355096212No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT157090.2599465677339606No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAATTTTCTCTTAA154580.2557931150316101No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG142900.23646549448840137No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA134570.22268132675510266No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC130700.21627739768813195No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG130000.2151190642651657No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC129600.21445715945204208No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG127140.210386444851332No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG123200.20386668244206468No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTACAGTCGCTACGACT121680.2013514441521951No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG120530.19944846781446476No Hit
CCGCTATCGACAGCAGCTATGGGAGAGCGCGCTCGTCGACATGCTAGAGT111830.18505203812902676No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGAGAGGG110720.1832152522726088No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT106850.1768113232056381No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG106410.17608322791120215No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG104410.17277370384558421No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG103520.17130096563638425No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA97570.1614551315411709No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA94750.1567887026086496No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG92520.15309858327548562No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG90750.15016965447741373No Hit
GGCGATACGACAGCAGTATCTGGATGGATCAGTATGGATCGACAAATGGC90200.1492595353593688No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC90070.14904441629510365No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA87240.14436143974225427No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT85120.14085334423269924No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCAT83110.13752727254675323No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG80550.13329108174276227No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGACAGAGATATCATGCAAG77790.12872393853220954No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC76730.126969890777432No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC73070.12091346173735121No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT67950.1124410801293693No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG65180.10785738929848845No Hit
GTACTGCTATGGGCGAGCGCTATGAGCGACTATGGGACCTATGGGAGAGC65090.10770846071553565No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCGCTAT63320.10477953191746377No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT62390.10324060322695143No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT62200.10292619844071774No Hit
CCGCTATCGACAGCAGTATCTGCTGTGTATGGGACTACAGTCGCTACGAC61790.10224774600726606No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG61100.10110596020462786No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT60450.10003036488330204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT1690300.0277.945041
TGATGAC2114000.0277.828435
GGCGATA1557700.0277.651
GATGACA2118400.0277.409246
GCGATAC1569350.0277.05392
TGACAGC2116700.0276.973728
GTACTGA2136950.0276.937741
ACTGATG2127500.0276.62223
GCTATCG1689850.0275.883423
CTGATGA2133650.0275.837924
TACTGAT2139450.0275.109652
CTATCGA1699450.0274.505374
CGATACG1586200.0273.891173
CGCTATC1714000.0273.565282
GATACGA1591500.0273.136634
TCGACAG1704700.0272.597357
ATGACAG2154300.0272.48247
CGACAGC3326200.0272.23588
ATACGAC1595550.0271.910645
TATCGAC1716150.0271.428165
Produced by FastQC (version 0.11.5)