FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492070
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCA403.6164838E-622.00000210
CCATGGT403.6164838E-622.0000023
CGAGGGA800.022.0000026
TGATAAC353.2160042E-522.010
CGGGGTG250.00258071922.04
GGCCACT250.00258071922.010
GGTGGCC250.00258071922.07
CAAGGGC250.00258071922.06
GTCGTTG302.8732145E-422.08
ACATGCA302.8732145E-422.01
TTACGTC250.00258071922.04
GGTGACG302.8732145E-422.07
GACGCCT353.2160042E-522.010
AGAGGGT250.00258071922.08
CAATACG353.2160042E-522.01
GAGCGAG900.020.7777793
TGAGCGA950.019.684212
AGCCGAA451.0022171E-519.5555558
TCCATGG408.990368E-519.2500022
AGTGGCC408.990368E-519.2500028