FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446307
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACGC302.8688466E-422.0000029
AGGTGGG605.9480953E-1022.0000028
GGCACGG250.00257791222.09
GTGGGGC250.00257791222.013
GACCGGG250.00257791222.09
CCGGTAC353.209497E-522.05
AGGTGCG250.00257791222.08
GTCGGAG2050.020.9268287
GATCCGC1000.020.96
GCGCCAC850.020.7058834
CTGAGCG2100.020.428571
CACGGGC551.2611599E-720.02
GACCGTG551.2611599E-720.01
TGGCTAG551.2611599E-720.03
TCTTGCC1050.019.904768
TCGGAGT2100.019.904768
CGGAGTG2100.019.904769
GGTGCTG501.1201864E-619.87
TTGGCTG459.996409E-619.5555559
GTAGCGC459.996409E-619.5555558