FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220758
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG302.86249E-422.0000029
GTCGCTC403.5934245E-622.0000027
TACTTCT605.9117156E-1022.0000029
GACGTTA302.86249E-422.0000021
AGCTTGT302.86249E-422.00000210
CGACCTA302.86249E-422.0000022
CTGAAAG302.86249E-422.0000023
CTACCCA656.730261E-1122.0000021
GGTGCAG302.86249E-422.0000027
GGGCACA302.86249E-422.0000025
TAGGCGA302.86249E-422.00000210
GAACCAG605.9117156E-1022.0000023
GACCTAG302.86249E-422.0000023
CCTCTGA302.86249E-422.0000026
CTAGCAT302.86249E-422.0000026
TCTGATG250.002573823322.08
GCCTTGG353.2000284E-522.09
GTTCTGA250.002573823322.022
CGAGTCC353.2000284E-522.06
CGAAAAG454.0506347E-722.03