FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948203
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAA16650.020.678688
GGATAGC16050.020.4922124
CTCGGAG2750.020.47
AGCCGAG3300.020.3333343
ACCGGGG8350.020.2874262
TAGCTGG13600.020.2205897
CGGGGTG8600.019.8255834
GTGTCCC14700.019.6802738
AGCTGGC13750.019.688
GGGGTGA8800.019.6255
TACCGGG10800.019.5555551
GGCGTAA14750.019.53898210
CGGATAG13100.019.4809153
GCTGGCT12950.019.4517369
CCCTCGA11650.019.4506449
CGGTGTC14950.019.424756
ACGCACG15500.019.3741931
CCGGGGT8750.019.363
ATCGGAT13550.019.3210331
GCAGACG2450.019.3061226
Produced by FastQC (version 0.11.5)