FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489588
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGCT656.730261E-1122.0000021
GTCGCGA555.2350515E-922.01
GCGACGC353.210317E-522.02
ACGGGCC353.210317E-522.02
TGGCAAG302.8693976E-422.05
GAGCGGT302.8693976E-422.03
GGGCCTG504.609865E-822.02
GCGGCAC250.00257826622.04
CGAGATC353.210317E-522.06
GTGGGAA801.8189894E-1220.6251
GGCCTAG651.6170816E-920.3076933
TCGGGAC501.1206412E-619.81
TTGATGG900.019.5555551
CAAGGGC459.999656E-619.5555556
GAAGGTC459.999656E-619.5555554
CATGCCG408.974661E-519.258
CCAGGTG805.2750693E-1119.256
AATGGCT408.974661E-519.251
GTGGAAT408.974661E-519.258
GCCTGAG408.974661E-519.253
Produced by FastQC (version 0.11.5)