FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6853314
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGA54450.021.2525256
GCGAGGG55350.020.8870835
GGAGTGG55100.020.66243210
CTGAGCG58050.020.6546081
GGGAGTG55200.020.6050749
GAGGGAG57100.020.4973727
GAGCGAG55300.020.4882453
TGAGCGA55700.020.3411122
AAGACGT35900.019.6100273
AGACGTT36650.019.508874
AGCGAGG60350.019.0289974
CTGGGCG22700.018.9955969
GGTGACG11250.018.871117
TTAGCTA37700.018.3527859
GAGTGGG13800.018.33333411
GACGCCT12900.018.33333410
TAGCTAC37800.018.30423410
CCGGACA15850.018.1135651
AGGGAGT65900.017.8937788
CGGACAG11150.017.7578492
Produced by FastQC (version 0.11.5)