FastQCFastQC Report
Fri 25 Sep 2020
000000000-CTV4D_l01_n01_JRS_019_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CTV4D_l01_n01_JRS_019_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043165
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAAG18600.019.0430093
AGGGCCT19750.018.491141
CAGGGGT10550.017.933653
GTGCTGG13200.017.9166661
TACCGGG15650.017.8530371
AGGGGTG7850.017.7961794
GCGGTGA4900.017.5102045
CCCTCTA22000.017.1510
AAGGGAA15300.017.111112
CGCTGCG10250.017.0634149
TCTGAGA18000.016.988898
GGCGAGC5700.016.9824564
GCGGAAA15450.016.8737871
GCCCGAA9800.016.724491
TCCAGAC20000.016.6655
GTCTGAG8300.016.5662657
CTGGTCC15200.016.55
GGAGATG16850.016.451041
GTGGAGA10500.016.447621
GCCTGGT13600.016.3382363
Produced by FastQC (version 0.11.5)