FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220839
Sequences flagged as poor quality0
Sequence length289
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG333200.7894165117409122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCA52300.0235.113867
TATCATC51400.0230.421229
CAGTATC99750.0125.116866
CGAGGAC2150.0125.0475855
TAAGCGA2150.0125.0475851
TGACAGC4800.0112.021798
GTTTGCA2900.0102.4663851
GCCCTCC8050.0101.951181
GCGAGGA2950.091.136374
GACAGCA147700.087.564051
CAACACG2900.082.9489753
GAGGACA3700.076.487146
ATGACAG6600.075.038517
GCAACAC3400.070.75062
TTGCATG4000.070.75063
AGGACAG3900.068.9364857
GCAGTAT186700.067.6052865
GCAGCTA1905.675247E-1059.5794535
AGCCTAT1804.0672603E-955.0282444
TATAGCC1052.8873736E-453.9052241
Produced by FastQC (version 0.11.5)