FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191105
Sequences flagged as poor quality0
Sequence length289
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG68459657.475705332443404No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT579224.862879427086613No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT565464.747356446325051No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT534684.488940941394755No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT269602.263444448642227No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT243312.042725032637761No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT160481.3473203453935632No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT149471.2548851696533891No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT141501.1879725129186764No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA25610.21501043149008692No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG24180.20300477287896532No Hit
GACAGAAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG24170.2029208172243421No Hit
CCTCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT18650.15657729587232025No Hit
CATCACTTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16030.1345809143610345No Hit
GACAGCAGTAGCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG14730.12366667926001487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCA207.608651E-8283.000031
AGCTAGG109.5861324E-4283.000033
TGAAAGC158.545881E-6283.08
GTTTGCA56450.0282.749331
GAGGACA59300.0282.522776
CGAGGAC58950.0282.519935
TTGCATG56950.0282.006133
TAAGCGA59500.0281.573121
GCGAGGA59400.0281.57074
GCTATCG27600.0281.461943
GTGCGAT14750.0281.081362
GATGACA40050.0280.526826
GCGATAC16400.0280.41162
AGGACAG59800.0279.92397
GGCGATA16600.0279.590361
GCAACAC63400.0279.429022
CGATGAC15050.0279.23925
TGATGAC25200.0278.507975
CAACACG64500.0278.173653
CGCTATC28000.0277.441072
Produced by FastQC (version 0.11.5)