FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799353
Sequences flagged as poor quality0
Sequence length289
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG68634685.86269145171157No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG69800.8732062055187133No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT44320.5544484101517102No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT37780.47263224132517173No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT33930.4244682887285092No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG24050.3008683272596712No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT19950.24957684527361504No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG15160.18965338217283226No Hit
CATCACTTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT12610.15775258240101683No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC11220.140363518995988No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10160.12710279438495883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACA158.54483E-6283.06
CTACTTA600.0283.02
AGTTTCA109.5855456E-4283.07
TACTTAG600.0283.03
GTCTGAC158.54483E-6283.05
CAGCCTA158.54483E-6283.03
ATCACTT1600.0283.02
CGAGGAC4150.0283.05
CTTGACA1600.0283.06
CGATGAC750.0283.05
GCAGCTA7350.0283.05
GGTCTGA158.54483E-6283.04
TATAATC750.0283.09
TGATGAC950.0282.999975
GCAACAC5550.0280.450442
GAGGACA4150.0279.590366
CAACACG5800.0278.12073
CAGCTAT7400.0277.263526
GCTATCG2350.0276.97873
GATGACA1750.0274.91436
Produced by FastQC (version 0.11.5)