FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479510
Sequences flagged as poor quality0
Sequence length289
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG39299181.95678922233112No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT71791.4971533440387061No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT65031.3561760964317742No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT47530.9912202039582074No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT35200.7340827094325457No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT32320.6740213968426102No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT19260.401660027945194No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15480.32282955517090367No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG13260.2765322933828283No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG12690.2646451585994036No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10330.2154282496715397No Hit
CATCACTTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9370.19540781214156117No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGTGCGCTCGTCGACTATGG8950.18664887072219558No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG6600.1376405080186023No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA4950.10323038101395174No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAGG109.58435E-4283.000033
GGTGACT109.58435E-4283.000038
AGGATCA207.6039214E-8283.000037
GAGGACA7000.0283.06
GCAACAC4700.0283.02
CGAGGAC7000.0283.05
GCGATAC1650.0283.02
GATGACA3350.0283.06
GTGCGAT1000.0283.02
TGATGAC2300.0283.05
AGGACAT158.5427055E-6282.999977
CAGCTAT158.5427055E-6282.999973
GGACATC158.5427055E-6282.999978
TTGCATG7400.0281.087863
GTTTGCA7300.0281.06161
TAAGCGA7100.0281.007021
GCTATCG3900.0279.37183
GCGAGGA7150.0279.041964
TGACAGC13000.0277.557688
CAACACG4800.0277.104163
Produced by FastQC (version 0.11.5)