FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009279
Sequences flagged as poor quality0
Sequence length289
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG88300287.48839518111444No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG29800.29526027986314984No Hit
GACAGAAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG22820.2261019995462107No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG12210.12097745023923019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAG109.585916E-4283.000032
ACTTAGA207.608105E-8283.000034
TACTTAG256.82121E-10283.03
GTATCAA158.545494E-6283.08
GCAGCTA750.0283.05
AGTATCA920950.0282.708077
TATCATC920650.0282.585029
GTATCAT926100.0281.09018
TCGCTGT400.0247.625029
CAGAGCG400.0247.625022
TGTATCA351.8189894E-11242.571437
TATAATC1000.0240.549999
CAGCCTA355.0986273E-9202.142853
TACAGCA5850.0193.504271
GAGGACA150.003226689188.666676
GATAGGA150.003226689188.666671
GATCATC256.5565975E-5169.89
GTATCAC256.5565975E-5169.88
AGGATCA256.5565975E-5169.87
TATCGTC256.5565975E-5169.89
Produced by FastQC (version 0.11.5)