FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758603
Sequences flagged as poor quality0
Sequence length289
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG28140537.095160446241316No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG117721.5517998215140199No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC75310.9927458763015701No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG64310.8477424950863627No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA64270.847215210063762No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG59920.78987296385593No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT56270.7417582055436112No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA53400.7039255051720069No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA53270.7022118288485545No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT52910.6974662636451477No Hit
GACAGCAGTATCATCGTATGGAGTCGACTACAGTCGCTACGACTATGGAG51720.6817795342227753No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG47840.6306328870305021No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT41980.5533856312194916No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG39220.5170029646600396No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT38220.5038208390950207No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC32710.43118732723176684No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG32580.4294736509083143No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC32370.42670540453966044No Hit
GACAGCAGTATCAGCAGTATGGAGTCGACAGCAGTATCTGCTGTCATGGA31520.41550059780939436No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT29780.3925636993262616No Hit
GACAGCAGTATCATATGGAGTCGAGAGCGCGCTCGTCGTCATGGAGTCGC29090.38346803268639856No Hit
TATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTAC28280.3727905109787333No Hit
GACAGCAGTATCATCGAGTCGAGAGCATAGACATCGAGTAGGACTCCGTA28240.37226322595613254No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT26160.34484440478089334No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC25690.33864880576533446No Hit
GACAGCAGTATCATCGACTATGGAGCTATGGAGTCGTCAGCAGTACTACT23740.31294366091354764No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT23030.3035843517623843No Hit
GACAGCAGTATCATCGAGACTGTATGGAGTCATGTATGGAGTCGACAGCA20130.2653561876238296No Hit
GACAGCAGTATCATCGTATGGAGTATGGAGTCGACTACAGTCGCTACGAC19270.2540195596379134No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA19120.25204224080316057No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG18740.2470330330884534No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT18510.24400114420849903No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT18320.24149654035114546No Hit
GACAGCAGTATCATCGAGAGTCATCGAGTCGAGAGCATAGACATCGTCTG18300.24123289783984508No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC17970.23688279640338888No Hit
GACAGCAGTATCATCGATACGTATACGTATGAGTATGGAGTATGGAGGAG17910.23609186886948771No Hit
GACAGCAGTATCACCCGAGTCGAGAGCGCGCTCGTCGACACACGACGACA17300.22805077227482626No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTATGGAGTCGTCAG16430.21658232303325983No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGAGTATGGAGT16020.2111776515516021No Hit
GACAGCAGTATCATCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGT15690.20682755011514586No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA15280.20142287863348812No Hit
GACAGCAGTATCATCAGCAGCAGTATGGAGTCGAGAGCATAGACATAGTC14870.19601820715183038No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT14130.18626343423371644No Hit
GACAGCAGTATCATGTCGATGGATGTCGATGATGATGGATCATGTCGATC14130.18626343423371644No Hit
GACAGCAGTATCATCGGAGTCGCGAGCGCGCTCGTCGACTATGGAGTCGT13860.18270426033116136No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG13220.1742676999695493No Hit
GACAGCAGTATCATCGAGTCGAGTATATGGAGTCGAGAGCATAGACATCC13120.1729494874130474No Hit
GACAGCAGTATCCTATGGAGTCACTATGGAGTCGTCAGCAGTACTACTGA12940.17057670481134402No Hit
GACAGCAGTATCATCGATGGATCCATCGCTACGACTATGGAGTCGACAGA12900.17004941978874327No Hit
GACAGCAGTATCATCGACAATCCATGGAGTCGACAGCAGTATCTGCTGTC12890.16991759853309307No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA12820.16899484974354173No Hit
GACAGCAGTATCATATGGAGTCGAGAGCATAGACACCACACACGACGACA12350.16279925072798287No Hit
GACAGCAGTATCATCGAGATATGGAGTCGAGAGCGCGCTCGTCGACTATG11810.1556809029228727No Hit
GACAGCAGTATCATCGATGAGCAGTCGCTACGACTATGGAGTCGACAGCA11770.15515361790027193No Hit
GACAGCAGTATCATGGAGTCGAGTCGACATAGTATGGAGTCGACTACAGT11720.15449451162202102No Hit
GACAGCAGTATCATGGTAAGTATGGAGTCGAGAGCGCGCTCGTCGACTAT11690.15409904785507045No Hit
GACAGCAGTATCATCGAGACGAGCGCGCTCTCGACTATGGAGTCGTCAGC11620.15317629906551913No Hit
GACAGCAGTATCATCGAGTCGAGAGCATAGACATCGAGTAGGACTCCATA11570.15251719278726816No Hit
GACAGCAGTATCATCGAGTCGTCAGCAGTACTACTGACGATGGAGTCGAC11510.15172626525336705No Hit
GACAGCAGTATCATCGCGAGCGATGATACTGACTAGAGAGTATCATGATC11510.15172626525336705No Hit
GACAGCAGTATCGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG11370.14988076767426442No Hit
GACAGCAGTATCATCGATATGGATGGAGTCGAGATCATCCAGCGCGCTCG11360.1497489464186142No Hit
GACAGCAGTATCATGATATATGACAGTATCTGCTGTCATGGAGTCGACTG11110.1464534150273595No Hit
GACAGCAGTATCATCGAGATGGATATGGAGTCGACAGAGATATCATGCAG10810.14249877735785385No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG10590.13959870973354968No Hit
GACAGCAGTATCATCGGACATGGACTATGGAGTCGTCAGCAGTACTACTG10490.13828049717704782No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC10250.13511678704144328No Hit
GACAGCAGTATCATCGTCGCGCATCATCATGGAGTCGAGAGCATAGACAT10140.13366675322929122No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG10000.13182125565018857No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9940.13103032811628743No Hit
GACAGCAGTATCATCATCTATGGAGTCGAGAGCGCGCTCGTCCTATGGAG9610.12668022667983123No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9560.12602112040158028No Hit
GACAGCAGTATCATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC9500.12523019286767914No Hit
GACAGCAGTATCATCGATGATCGAGAGCATAGACATCTGCATATGGAGCA9370.1235165165442267No Hit
GACAGCAGTATCATCAGTATGGAGTCGACAGCAGTATCTGCTGTCATGGA9330.12298923152162594No Hit
GACAGCAGTATCATCCTATGGAGTCGCGAGCGCTATGACTCCATAGGATC9190.1211437339425233No Hit
GCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACT9070.11956187887472104No Hit
GACAGCAGTATCATCGAGTCGACTACAGTCGCTAATTCCAAATTCCGATT9060.11943005761907083No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG9050.11929823636342066No Hit
CATCACTTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8930.11771638129561839No Hit
GACAGCAGTATCATCAGCAGCAGTATGGAGTCGAGAGCGCGCTCGTCGAC8840.1165299899947667No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCCTATGGAGTCGTCAG8720.11494813492696444No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCATAGACATCGAGTATGGAGT8660.11415720739306331No Hit
GACAGCAGTATCATCGATGGAGTCGACAGCAGTGTGTGAGTCTATGGAGT8590.11323445860351199No Hit
TGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTACTG8590.11323445860351199No Hit
AGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGAGATATC8240.10862071465575539No Hit
GACAGCAGTATCATCGATACGATCGATGGAGTCGTCAGCAGTACTACTGA8200.10809342963315463No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA8000.10545700452015086No Hit
GACAAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGAGA7930.10453425573059953No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG7780.10255693689584672No Hit
GACAGCAGTATCATCGATGGAGTCGTCAGCAGTACTACTGACGATGGAGT7770.10242511564019653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCA568600.0269.885257
TATCATC533900.0268.158269
TATCCTA21750.0232.905759
AGTATCC23100.0227.870157
GCGAGGA5350.0227.457954
GTATCCT23300.0222.877678
GTATCAC10250.0218.117088
TAAGCGA5800.0209.810351
TATCGGA3500.0202.142859
TTGCATG2950.0201.457633
CGAGGAC6100.0199.49185
CAGCCTA13700.0199.339423
GAGGACA6050.0198.801656
GTTTGCA3000.0198.11
GTATCAG8300.0197.759058
GCAACAC4300.0197.441852
GTATCAT757800.0197.349378
TGACAGC9650.0196.487058
AGCGAGG6250.0194.704013
ACAGCCT14400.0189.649292
Produced by FastQC (version 0.11.5)