FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916191
Sequences flagged as poor quality0
Sequence length289
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG71222837.16894610192825No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG485242.5323154111463837No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA301011.5708768071658825No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG290111.5139931249024758No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG273241.4259538845553497No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC272641.4228226726876392No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA243481.27064577591691No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT235581.2294181529920556No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA235051.226652249175578No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT211661.1045871731993313No Hit
TATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTAC191600.999900323088878No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG145150.7574923376636254No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG138910.7249277342394365No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC131460.6860485202153648No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC129020.6733149252866755No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT106600.5563119751632275No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT97440.5085088073161809No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA89760.46842929540948686No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA74970.39124492287042373No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC69950.3650471169105794No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC68360.3567494054611466No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA65320.34088459866474685No Hit
GCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACT63770.3327956346731615No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG56630.2955342134474069No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC50810.2651614583306153No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG49660.25915996891750354No Hit
GACAGCAGTATCATCGAATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG46650.24345172271448934No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG44620.23285778922873557No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG44140.23035281973456714No Hit
GACAGCAGTATCATCGACTATGGAGTCCTATGGAGTCGTCAGCAGTACTA40800.21292240700431223No Hit
TGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTACTG40590.2118264828506135No Hit
GACAGCAGGTCTATGGAGTCGTATGGAGTCGACTACAGTCGCTACGACTA37730.19690103961452696No Hit
GACAGCAGTATCATCGACTATGGAGCTATGGAGTCGTCAGCAGTACTACT37590.19617042351206115No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCCTATGGAGTCGTCAG36160.18870770189401787No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG36120.1884989544361705No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGGTAT33660.17566098577855754No Hit
GACAGCAGTATCATCGAGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG29080.15175940185503428No Hit
GACAGCAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGA28770.1501416090567172No Hit
AGTATGGAGTCGACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACA27260.14226139252297917No Hit
GACAGCAGCTATGGAGTCACTATGGAGTCGTCAGCAGTACTACTGACGAT26500.13829519082387925No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25960.13547710014293984No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCCTATGGAGTCG25670.13396368107354642No Hit
GACAGCAGTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC25540.1332852518355425No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTAAGT25140.13119777725706883No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG24950.13020622683229385No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA24190.12624002513319393No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCCTATGGAGTCGTCAGCA23480.12253475775640321No Hit
GACAGCAGTATCATCGACTATGGAGTCTATGGAGTCGTCAGCAGTACTAC23360.1219085153828611No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT23060.12034290944900586No Hit
GACAGCAGTATCATCCTATGGAGTCGTCAGCAGTACTACTGACGATGGAG22910.11956010648207825No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCAGTATGGAGTC22630.1180988742771467No Hit
GACAGCAGTATCATCGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT22180.11575046537636384No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGACTATGGAGTCGTCAGCAGT20220.10552183994184297No Hit
GACAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA20080.10479122383937718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC1154550.0277.680949
AGTATCA1208250.0277.284947
TATCCTA68900.0262.052259
GTATCCT72850.0250.368568
AGTATCC75000.0249.794687
GTATCAC22500.0247.782238
GTATCAT1430050.0228.81638
TATCACT23700.0228.668789
CAGCCTA56900.0225.55453
GCAGGTC5100.0224.73535
ACAGCCT57650.0223.111022
GCAGCTA30900.0214.76865
GACACTA44800.0212.881681
CAGGTCT5600.0202.142856
GACAGCC65250.0199.075851
ACAGCTA62400.0196.603362
AGCAGCT34700.0192.064834
GACAGCT64850.0190.266771
ACAGCAC55000.0189.352722
CAGCACT55050.0188.666673
Produced by FastQC (version 0.11.5)