FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304011
Sequences flagged as poor quality0
Sequence length289
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG24730181.3460697145827No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT31761.0446990404952452No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT27340.8993095644565493No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16720.5499800994042979No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16020.5269546167737351No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT13530.44504968570216213No Hit
CATCACTTGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT12110.3983408495087349No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11580.38090726980273737No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11160.36709198022439976No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG8490.27926621076211056No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8360.27499004970214896No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG5420.17828302265378557No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG4320.142100121377187No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT4280.14078437951258344No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG4130.13585034752032No Hit
GACAGCAGTATCATCGACTATGGAGTCTATGGAGTCGTCAGCAGTACTAC3770.12400867073888773No Hit
GACAGCAGTATCATCAACCGCTCACCGCTCATCGACTATGGAGTCGTCAG3610.1187457032804734No Hit
GAAAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3420.1124959294236064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAC1250.0283.000035
GTTTGCA1650.0283.01
GAGGACA3250.0283.06
GACAGGA109.5826294E-4283.01
GCAACAC1700.0283.02
CAACACG1750.0283.03
CTTGACA1550.0283.06
CAGGAGT109.5826294E-4283.03
TCACTTG1500.0283.03
GCGATAC1150.0283.02
CGATGAC1100.0283.05
CGGTATC207.599374E-8283.06
GGCGATA1150.0283.01
TAAGCGA3250.0283.01
GCGAGGA3250.0283.04
TACTTAG1050.0282.999973
GCTATCG2350.0282.999973
ACTTAGA1050.0282.999974
CGAGGAC3300.0278.712135
CTATCGA2550.0277.450964
Produced by FastQC (version 0.11.5)