FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n02_WGS15-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n02_WGS15-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643168
Sequences flagged as poor quality0
Sequence length289
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG54738385.10731255286332No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT44320.689089009403453No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT44210.6873787253097169No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT33170.515728394447485No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT31020.48230011443355386No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT28430.44203069804467887No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT27600.4291258271555799No Hit
GACAGCAGTATCATCGACTATGGAGTCGAAAGCGCGCTCGTCGACTATGG18770.2918366585402259No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15970.2483021543360366No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15290.2377294890293049No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT15060.23415344046967512No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG9580.1489501965271904No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG8390.13044803224040996No Hit
GAAAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG8390.13044803224040996No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG8190.1273384247972536No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG7700.11971988656152048No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA7120.11070202497636698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTA1000.0283.02
AGTTTCA158.544055E-6283.07
TACTTAG1000.0283.03
GATCATC256.8030204E-10283.09
ATCACTT450.0283.02
ACTTAGA1000.0283.04
GTATGAT109.5851097E-4283.08
TCGCTGT207.605922E-8283.09
CGAGGAC4800.0283.05
CAGTGCC109.5851097E-4283.06
TCACTTG450.0283.03
TTGCATG2800.0283.03
CGGTATC158.544055E-6283.06
AGGATCA256.8030204E-10283.07
TATAATC305.456968E-12283.09
TATCGTC109.5851097E-4283.09
TCTATCG350.0283.03
GCTATCG4350.0279.747133
CAACACG4150.0279.590363
GCGATAC3150.0278.507932
Produced by FastQC (version 0.11.5)