FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220839
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTG100.007239937143.999886
CATGTGG2800.0133.714179
AATGTGG4800.0127.4998869
AGTGTGG4400.0122.727169
CGTGTGG3700.0116.7566459
TCTGTGG5200.0116.307599
ACTGTGG3950.0109.3679
CCCGTGT203.9748737E-4107.999917
CACTGTG850.0101.646978
ACGTGTG1200.095.999918
TGTGTGG7650.095.058749
CGTTGTG1750.094.628498
AACTGTG1300.088.61538
TCATGTG1650.087.2726448
CCGTGTG1000.086.3999258
CCTTGTG1100.085.090838
AAGTGTG2150.083.7208568
CGCTGTG950.083.368358
CGCGTGT353.558649E-582.2856457
ACTTGTG1500.081.599938
Produced by FastQC (version 0.11.5)