FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191105
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC351.2732926E-11144.000023
GGGGATA253.8684448E-8144.000022
TTCTACG351.2732926E-11144.000022
TTAGGGC351.2732926E-11144.000023
AGGTAGA253.8684448E-8144.000022
GAGGAAC351.2732926E-11144.000023
AGCAAAA253.8684448E-8144.000021
GATACGT700.0144.000025
CCCCTGG253.8684448E-8144.000021
TCGCAGA253.8684448E-8144.000023
CCGGGGT351.2732926E-11144.000025
CGAACCT500.0144.000023
TCACCGT253.8684448E-8144.000025
AAATATG1000.0144.000026
GTACAGG351.2732926E-11144.000021
CTTCCAG500.0144.000023
AGATAAG253.8684448E-8144.000024
AGCCCTT351.2732926E-11144.000022
CTATCCG253.8684448E-8144.000022
AGCCCTA253.8684448E-8144.000024