FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799353
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAG151.2642902E-4144.000021
AGCACCC600.0144.000021
AGCACAG306.82121E-10144.000022
AGCACAC151.2642902E-4144.000024
TCGCCTT151.2642902E-4144.000021
ATTTAAA600.0144.000021
CGGGTTA306.82121E-10144.000022
TATCACA306.82121E-10144.000021
CTAGAGG151.2642902E-4144.000024
AACGTAG306.82121E-10144.000024
CGTGGGC306.82121E-10144.000024
CTAGAAC151.2642902E-4144.000024
ATGGGAG600.0144.000021
CTATGCA306.82121E-10144.000021
GAGTGGG306.82121E-10144.000024
GGAATAA151.2642902E-4144.000022
CGGAACT151.2642902E-4144.000025
AGGTAAT151.2642902E-4144.000025
CCCCTTG306.82121E-10144.000026
AACCGAT151.2642902E-4144.000025