FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479510
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATCGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA7390.1541156597359805No Hit
TCTAGATTTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6550.13659777689724928No Hit
ATCGAACCTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6300.13138412129048402No Hit
AACCGTTATGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6280.1309670288419428No Hit
TCCTATACAATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6190.12909011282350732No Hit
ATATCTTGCATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6180.12888156659923672No Hit
ACGCTAAGTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6120.12763028925361308No Hit
AAGTATTAAATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6110.12742174302934245No Hit
ATTAATGGTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6100.12721319680507184No Hit
TACTTTAGTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6090.12700465058080124No Hit
GTCCTAGCGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6080.12679610435653063No Hit
TGGTGTAGTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6080.12679610435653063No Hit
TTTGTTCCTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6080.12679610435653063No Hit
AAACTGGTTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6050.12617046568371879No Hit
TGTGACGGCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA6010.12533628078663636No Hit
TGTTTTTCGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5950.1240850034410127No Hit
GCCCAGACCATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5930.12366791099247149No Hit
TAAGCCTTTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5920.12345936476820087No Hit
GGTTCGGTTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5860.12220808742257723No Hit
TGGTTGATTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5830.12158244874976538No Hit
TCTTTGATTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5810.12116535630122417No Hit
ATCTTAAAGTTGTGGATGAATACTGCCATTTGTCGATCCATACCGCATGA5790.12074826385268295No Hit
CTTCTAATCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5730.1194969865070593No Hit
GTATGTACGCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5720.11928844028278869No Hit
GAACTTATGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5710.11907989405851807No Hit
GTTCTGGTTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5710.11907989405851807No Hit
CATGCGGGAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5680.11845425538570624No Hit
TAGTTAAAACTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5680.11845425538570624No Hit
CCCGGACTGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5680.11845425538570624No Hit
CTATGGTTTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5670.11824570916143563No Hit
ACTGTTCATGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5660.11803716293716503No Hit
CAGTTATCATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5650.11782861671289441No Hit
ATCTTTGCTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5620.11720297804008258No Hit
TGTATATCTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5610.11699443181581197No Hit
CTTTTTAAATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5580.11636879314300015No Hit
TACGATGACGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5550.11574315447018832No Hit
TAATATGTGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5550.11574315447018832No Hit
CGGGACGGATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5550.11574315447018832No Hit
TATATACCCCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5520.11511751579737649No Hit
ATAATGCTCATGTGGATGAATACTGCCATTTGTCGATCCATACTGCATGA5510.11490896957310588No Hit
GTCTAGAGGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5510.11490896957310588No Hit
ACTGAAGCTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5470.11407478467602343No Hit
TAGGTACGCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5460.11386623845175284No Hit
GAGGGCCGACTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5450.11365769222748223No Hit
TCTGTAGCATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5430.113240599778941No Hit
TTTGCAACTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5420.1130320535546704No Hit
ATTTTTCCCGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5390.11240641488185857No Hit
GATTGTGATTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5380.11219786865758795No Hit
TGCAGATCTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5370.11198932243331734No Hit
TCTATGTGGCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5370.11198932243331734No Hit
CTTATAATTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5370.11198932243331734No Hit
TTTATAGCTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5360.11178077620904672No Hit
GTCTCTTGAATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5360.11178077620904672No Hit
GGATCACTCGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5340.11136368376050551No Hit
GGCTGGGATATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5330.11115513753623489No Hit
ACTATTGTAATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5330.11115513753623489No Hit
GGCTCGTTGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5310.11073804508769369No Hit
TTCGGCTGTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5310.11073804508769369No Hit
TGAGTGATCATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5300.11052949886342309No Hit
TTTCATAGGGTGTGGATGAATACTGCCATTTGTCGATCCATACCGCATGA5290.11032095263915247No Hit
TCAGTGTACGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5290.11032095263915247No Hit
TTTCTAAGTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5290.11032095263915247No Hit
TATGAGATTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5260.10969531396634064No Hit
GCGCCCTGTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5260.10969531396634064No Hit
CGGAATACTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5250.10948676774207003No Hit
ATCGGTTCACTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5240.10927822151779942No Hit
TATAATATGCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5230.1090696752935288No Hit
AGGATTTGGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5200.10844403662071697No Hit
CTTGAACACGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5190.10823549039644638No Hit
ATGCACCTGGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5190.10823549039644638No Hit
TCGACGATAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5190.10823549039644638No Hit
AGGGCGATGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5180.10802694417217577No Hit
TTTGACTTATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5160.10760985172363455No Hit
TCGGTAAGCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5150.10740130549936394No Hit
CGACTTAATGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5130.10698421305082272No Hit
TTAGTCTAACTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5100.10635857437801088No Hit
CTTGCGACCATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5100.10635857437801088No Hit
ATTCCACTGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5080.10594148192946966No Hit
GGTAGCATCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5070.10573293570519905No Hit
AGCATAGTCGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5060.10552438948092843No Hit
TACTTCAGAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5040.10510729703238723No Hit
AATTACAATATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5020.10469020458384601No Hit
GAGTACACTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5020.10469020458384601No Hit
TCAGCTTTGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5020.10469020458384601No Hit
TAAGTAATTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5010.1044816583595754No Hit
TTAACCTAGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5010.1044816583595754No Hit
CTTTTTTTGGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5010.1044816583595754No Hit
TACGTTTGGATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5000.1042731121353048No Hit
GTAGCACGTGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA5000.1042731121353048No Hit
GAAGGAATTTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4980.10385601968676357No Hit
CATGTCAGGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4980.10385601968676357No Hit
TTTTAGTACGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4980.10385601968676357No Hit
TCGTGAAATATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4960.10343892723822234No Hit
GTGTTTGGACTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4960.10343892723822234No Hit
CCATGTCGAATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4950.10323038101395174No Hit
GCCACTTTCTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4930.10281328856541051No Hit
TGTAGGTCTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4930.10281328856541051No Hit
CTTGTTTACTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4910.10239619611686931No Hit
GGCATATGGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4900.10218764989259871No Hit
TTCATCATAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4880.10177055744405748No Hit
ATTGGCTGGTTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4870.10156201121978686No Hit
TGTACATATTTGTGGATGAATACTGCCATTTGTCGATCCATACCGCATGA4840.10093637254697503No Hit
TAAAGAGTTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4840.10093637254697503No Hit
CAACATCGTCTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4830.10072782632270443No Hit
GAGGGTTTAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4830.10072782632270443No Hit
AATTTATGAGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4830.10072782632270443No Hit
TGAACCGCTATGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4820.1005192800984338No Hit
TAGCATTTGGTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4800.1001021876498926No Hit
CGTGATACATTGTGGATGAATACTGCCATTTGTCGATCCATAGTCGCTCA4800.1001021876498926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC151.2639772E-4144.000023
GTGTTCT1150.0144.000025
ATTTACA151.2639772E-4144.000023
CTAGATT600.0144.000022
CGGGTTG306.82121E-10144.000026
CTAGAGG600.0144.000023
CGGAATA600.0144.000021
CGGGTGG306.82121E-10144.000024
GTGCAAA306.82121E-10144.000024
AACGTCT151.2639772E-4144.000024
CATGGCT151.2639772E-4144.000023
TGGAGTT600.0144.000021
CTATGGG151.2639772E-4144.000022
CTAGAAC151.2639772E-4144.000021
GAAATAG151.2639772E-4144.000023
CTATGCG151.2639772E-4144.000022
GGGGAGT151.2639772E-4144.000025
TAGAGAG306.82121E-10144.000024
TACCCTG151.2639772E-4144.000026
TCGTTCG306.82121E-10144.000022
Produced by FastQC (version 0.11.5)