FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009279
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGTG450.0144.000026
TGGACGT450.0144.000025
CACGATG900.0144.000026
TAACGGT450.0144.000025
CCTTTGA450.0144.000021
CGCAGTG450.0144.000026
GCGAGAG100.0072391066144.02
CCCTGCT151.2643881E-4144.05
AGCACAG100.0072391066144.02
AGCACAC100.0072391066144.01
TCGCCTA202.205279E-6144.03
CTGCTAG151.2643881E-4144.04
TATCACA151.2643881E-4144.02
GTGCAAA253.868081E-8144.01
AACCGTG1150.0144.06
GAAATCC100.0072391066144.03
CTATGCG100.0072391066144.04
TTAGGCG100.0072391066144.02
CGGAACT151.2643881E-4144.05
GGGTGGC100.0072391066144.03
Produced by FastQC (version 0.11.5)