FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758603
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGG253.8671715E-8144.000021
AGCGTCT253.8671715E-8144.000025
AACAAAC253.8671715E-8144.000023
ACAAACG253.8671715E-8144.000024
GGAGCAA253.8671715E-8144.000022
GAGCTCC253.8671715E-8144.000024
CAAGTAC253.8671715E-8144.000024
GTACAAA253.8671715E-8144.000022
CTTCCGT500.0144.000025
CCTAGGG253.8671715E-8144.000024
CAGTTAG253.8671715E-8144.000022
CTATACG253.8671715E-8144.000023
TAGCGGA253.8671715E-8144.000024
GACCGAG253.8671715E-8144.000024
GGCAAAG253.8671715E-8144.000021
TCTTAGG253.8671715E-8144.000023
CCCAGTG253.8671715E-8144.000026
ATGCTAG253.8671715E-8144.000021
AGTGGGT253.8671715E-8144.000025
AGGGTAC253.8671715E-8144.000023