FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916191
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG351.2732926E-11144.000024
CAGCGGG351.2732926E-11144.000024
CCTTACA351.2732926E-11144.000021
CTATCCG351.2732926E-11144.000021
CCAGGAG351.2732926E-11144.000024
ACCCTGG351.2732926E-11144.000021
ACGGGAC351.2732926E-11144.000022
CCCGTAA351.2732926E-11144.000022
AACTGGG351.2732926E-11144.000022
ACCACGG351.2732926E-11144.000022
CGCACAG351.2732926E-11144.000022
CACGTCC351.2732926E-11144.000023
CACCGAT351.2732926E-11144.000025
CCCGGTG351.2732926E-11144.000026
ACGACCT351.2732926E-11144.000025
TCCGGCA151.264564E-4144.03
AGCACCC253.8693543E-8144.03
ACAACGG100.0072396104144.02
ACAACCC151.264564E-4144.01
CAGGTCA151.264564E-4144.02
Produced by FastQC (version 0.11.5)