FastQCFastQC Report
Mon 24 Feb 2020
000000000-CPFB7_l01_n01_WGS15-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CPFB7_l01_n01_WGS15-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643168
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG151.2641762E-4144.000022
AGGTCAC151.2641762E-4144.000023
TCCGGAC151.2641762E-4144.000023
TCACGAT306.82121E-10144.000025
TCGCCTG306.82121E-10144.000026
GCCCCGG306.82121E-10144.000021
ATCCTGG151.2641762E-4144.000023
GTTTGCA151.2641762E-4144.000022
ATCCTAT151.2641762E-4144.000025
AGACTTC151.2641762E-4144.000023
TTCTAGT151.2641762E-4144.000025
CAGGTCC151.2641762E-4144.000021
TAGAGTA151.2641762E-4144.000024
AGCAATT306.82121E-10144.000022
CGGGTCT600.0144.000025
TGTCAAG151.2641762E-4144.000023
CGGGTAT151.2641762E-4144.000025
CGTGGCT306.82121E-10144.000022
GGCAGTT306.82121E-10144.000024
CAGCGGG306.82121E-10144.000022