FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717259
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.2151245226619673No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG9070.1264536241441376No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7860.10958384628146876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG1950.085.51096145
CGTCTCG902.8194336E-1064.443058
CAGTATC3150.062.141516
AGTCTCG851.304943E-859.7045868
GGAGTCC2150.057.3283352
GGAGTAC1154.0017767E-1156.741862
GCAGTAT3600.052.3599785
GTCTCGT2300.050.4336939
AAGCCTA1056.9001544E-848.335662
GAGTACG1053.82134E-641.4305653
AGTCCGC907.4812764E-540.2769054
CAGAGTA2700.037.591778145
GCCTAGT1406.607261E-736.2492184
GGAGACC1458.6905857E-735.0016822
AGGAGGC2950.034.4204331
GACAGCA5700.034.3557321
GTCCGGC850.002741762434.1169055
CTGCCCG1501.1328721E-633.834963
TATCATC5000.033.3492779
AGTATCA5800.032.49937
Produced by FastQC (version 0.11.5)